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Quantitative proteomics in biological research
Author(s)
Date Issued
2009-10
Date Available
2013-04-15T11:43:10Z
Abstract
Proteomics has enabled the direct investigation of biological material, at first through the analysis of individual proteins, then of lysates from cell cultures, and finally of extracts from tissues and biopsies from entire organisms. Its latest manifestation - quantitative proteomics - allows deeper insight into biological systems. This article reviews the different methods used to extract quantitative information from mass spectra. It follows the technical developments aimed toward global proteomics, the attempt to characterize every expressed protein in a cell by at least one peptide. When applications of the technology are discussed, the focus is placed on yeast biology. In particular, differential quantitative proteomics, the comparison between an experiment and its control, is very discriminating for proteins involved in the process being studied. When trying to understand biological processes on a molecular level, differential quantitative proteomics tends to give a clearer picture than global transcription analyses. As a result, MS has become an even more indispensable tool for biochemically motivated biological research.
Type of Material
Journal Article
Publisher
Wiley
Journal
Proteomics
Volume
9
Issue
20
Start Page
4590
End Page
4605
Copyright (Published Version)
2009 WILEY-VCH Verlag GmbH & Co.
Language
English
Status of Item
Peer reviewed
ISBN
1615-9861 (Electronic)
This item is made available under a Creative Commons License
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