Quantitative proteomics in biological research

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dc.contributor.author Wilm, Matthias
dc.date.accessioned 2013-04-15T11:43:10Z
dc.date.available 2013-04-15T11:43:10Z
dc.date.copyright 2009 WILEY-VCH Verlag GmbH & Co. en
dc.date.issued 2009-10
dc.identifier.citation Proteomics en
dc.identifier.isbn 1615-9861 (Electronic)
dc.identifier.uri http://hdl.handle.net/10197/4247
dc.description.abstract Proteomics has enabled the direct investigation of biological material, at first through the analysis of individual proteins, then of lysates from cell cultures, and finally of extracts from tissues and biopsies from entire organisms. Its latest manifestation - quantitative proteomics - allows deeper insight into biological systems. This article reviews the different methods used to extract quantitative information from mass spectra. It follows the technical developments aimed toward global proteomics, the attempt to characterize every expressed protein in a cell by at least one peptide. When applications of the technology are discussed, the focus is placed on yeast biology. In particular, differential quantitative proteomics, the comparison between an experiment and its control, is very discriminating for proteins involved in the process being studied. When trying to understand biological processes on a molecular level, differential quantitative proteomics tends to give a clearer picture than global transcription analyses. As a result, MS has become an even more indispensable tool for biochemically motivated biological research. en
dc.language.iso en en
dc.publisher Wiley en
dc.rights This is the author's version of the following article: Wilm, M. (2009), Quantitative proteomics in biological research. Proteomics, 9: 4590–4605 which has been published in final form at http://dx.doi.org/10.1002/pmic.200900299 en
dc.subject MS en
dc.subject Protein interaction en
dc.subject Affinity chromatography en
dc.subject Proteomics methods en
dc.subject Functional proteomics en
dc.subject Cell biology en
dc.title Quantitative proteomics in biological research en
dc.type Journal Article en
dc.internal.authorcontactother matthias.wilm@ucd.ie
dc.internal.availability Full text available en
dc.internal.webversions http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=19743428
dc.status Peer reviewed en
dc.identifier.volume 9 en
dc.identifier.issue 20 en
dc.identifier.startpage 4590 en
dc.identifier.endpage 4605 en
dc.identifier.doi 10.1002/pmic.200900299
dc.neeo.contributor Wilm|Matthias|aut|
dc.description.admin AMS en
dc.internal.rmsid 133034095
dc.date.updated 2013-02-23T18:47:59Z


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