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Protein adhesion on water stable atmospheric plasma deposited acrylic acid coatings
Author(s)
Date Issued
2013-11-15
Date Available
2013-09-27T12:16:43Z
Abstract
There is considerable interest in the application of plasma polymerised acrylic acid (ppAAc) coatings due to their ability to enhance the adhesion of cells and proteins. An issue with this coating however is its stability in water and previous studies carried out using low pressure plasmas have demonstrated that high plasma powers are required to achieve water stable coatings. In this paper the use of both helium and air atmospheric plasmas is compared for the deposition of ppAAc coatings. The deposition studies were carried out on silicon wafer substrates using the PlasmaStreamâ„¢ and PlasmaTreatâ„¢ plasma jet deposition systems respectively. The coatings were characterised using contact angle, FTIR, SEM, XPS, ellipsometry and optical profilometry. While both the helium and air plasmas were successful in the deposition of ppAAc coatings, the nm thick films deposited using the PlasmaTreat system exhibited significantly higher levels of water stability, probably due to a higher level of coating cross-linking. Ellipsometry measurements demonstrated only a 0.2 nm reduction in the thickness of an 18 nm thick ppAAc coating, when immersed in an aqueous buffer solution for one hour. Protein attachment studies were carried out using a flow cell system, which was monitored using a spectroscopic ellipsometer. This study was carried out with Bovine Serum Albumin (BSA), Immunoglobulin G (IgG) and Fibrinogen (Fg) proteins. In all three cases increased levels of protein adhesion were observed for the ppAAc coating, compared to that obtained on the uncoated silicon wafer substrates.
Type of Material
Journal Article
Publisher
Elsevier
Journal
Surface and Coatings Technology
Volume
234
Issue
15 November 2013
Start Page
53
End Page
59
Copyright (Published Version)
2013 Elsevier
Language
English
Status of Item
Peer reviewed
This item is made available under a Creative Commons License
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Protein adhesion on water stable atmospheric plasma done.pdf
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6.67 MB
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