Bi-directional efects of vitamin B12 and methotrexate on Daphnia magna ftness and genomic methylation

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dc.contributor.author Kusari, Fitore
dc.contributor.author O'Doherty, Alan
dc.contributor.author Hodges, Nikolas J.
dc.contributor.author Wojewodzic, Marcin W.
dc.date.accessioned 2018-05-14T13:00:43Z
dc.date.available 2018-05-14T13:00:43Z
dc.date.copyright 2017 the Authors en
dc.date.issued 2017-09-19
dc.identifier.citation Scientific Reports en
dc.identifier.uri http://hdl.handle.net/10197/9381
dc.description.abstract Here we interrogated, using three separate but complementary experimental approaches, the impact of vitamin B12 availability and methotrexate exposure on Daphnia magna, which we hypothesised should have an opposite efect on One carbon metabolism (OCM). OCM is a vital biological process supporting a variety of physiological processes, including DNA methylation. Contrary to mammalian models, this process remains largely unexplored in invertebrates. The purpose of this study was to elucidate the impact of OCM short-term alteration on the ftness and epigenome of the keystone species, Daphnia. We used maternal age at reproduction, brood size and survival rates in combination with DNA methylation sensitive comet assay to determine the efects of vitamin B12 or MTX on ftness and the epigenome. Vitamin B12 had a positive infuence on Daphnia ftness and we provide evidence demonstrating that this may be associated with an increased level of genome-wide DNA methylation. Conversely, exposing D. magna to MTX negatively infuenced the ftness of the animals and was associated with loss of global DNA methylation, translating in decreased ftness. These results highlight the potential importance of OCM in invertebrates, providing novel evidence supporting a potential role for epigenetic modifcations to the genome in D. magna environmental adaptability. en
dc.description.sponsorship European Commission - Seventh Framework Programme (FP7) en
dc.language.iso en en
dc.publisher Springer en
dc.rights This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. Te images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. en
dc.subject Epigenetics en
dc.subject DNA en
dc.subject Methylation en
dc.subject Daphnia en
dc.subject Freshwater ecology en
dc.subject Ecology en
dc.title Bi-directional efects of vitamin B12 and methotrexate on Daphnia magna ftness and genomic methylation en
dc.type Journal Article en
dc.internal.authorcontactother alan.odoherty@ucd.ie
dc.status Peer reviewed en
dc.identifier.volume 7 en
dc.identifier.doi 10.1038/s41598-017-12148-2
dc.neeo.contributor Kusari|Fitore|aut|
dc.neeo.contributor O'Doherty|Alan|aut|
dc.neeo.contributor Hodges|Nikolas J.|aut|
dc.neeo.contributor Wojewodzic|Marcin W.|aut|
dc.description.othersponsorship Norwegian Research Council en
dc.internal.rmsid 807360099
dc.date.updated 2017-09-19T13:44:26Z


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