Monopoli, Marco P.

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Monopoli, Marco P.
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Monopoli, Marco P.

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  • Publication
    In depth characterisation of the biomolecular coronas of polymer coated inorganic nanoparticles with differential centrifugal sedimentation
    (Springer, 2021-03-19)
    Perez-Potti, André 
    ;
    Lopez, Hender 
    ;
    Pelaz, Beatriz 
    ;
    Kelly, Philip 
    ;
    Dawson, Kenneth A. 
    ;
    Krpetic, Zeljka 
    ;
    Monopoli, Marco P. 
    ;
    et al. 
    Advances in nanofabrication methods have enabled the tailoring of new strategies towards the controlled production of nanoparticles with attractive applications in healthcare. In many cases, their characterisation remains a big challenge, particularly for small-sized functional nanoparticles of 5 nm diameter or smaller, where current particle sizing techniques struggle to provide the required sensitivity and accuracy. There is a clear need for the development of new reliable characterisation approaches for the physico-chemical characterisation of nanoparticles with significant accuracy, particularly for the analysis of the particles in the presence of complex biological fluids. Herein, we show that the Differential Centrifugal Sedimentation can be utilised as a high-precision tool for the reliable characterisation of functional nanoparticles of different materials. We report a method to correlate the sedimentation shift with the polymer and biomolecule adsorption on the nanoparticle surface, validating the developed core-shell model. We also highlight its limit when measuring nanoparticles of smaller size and the need to use several complementary methods when characterising nanoparticle corona complexes.
      241Scopus© Citations 13
  • Publication
    Synthesis, characterization and programmable toxicity of iron oxide nanoparticles conjugated with D-amino acid oxidase
    (Royal Society of Chemistry, 2017-01-05)
    Balzaretti, Riccardo 
    ;
    Meder, Fabian 
    ;
    Monopoli, Marco P. 
    ;
    Boselli, Luca 
    ;
    et al. 
    D-amino acid oxidase (DAAO) is an enzyme which generates reactive oxygen species (ROS) and it is believed to have potential uses as a novel therapeutic molecule if internalized by cancer cells or if they are localized close to their plasma membrane. When conjugated onto iron oxide nanoparticles (NPs), the enzyme can be magnetically directed to targeted locations with an increased efficacy. A subsequent injection of DAAO substrate D-alanine can initiate ROS production and induce apoptosis of cells surrounding the NP-DAAO complex. Here, we describe a platform for optimal bioconjugation using monodisperse γ-Fe2O3 NPs (∼10 nm) resulting in high DAAO loading, stable NP-DAAO dispersions and more than 90% enzymatic activity recovery, which is retained using the particles in human serum. Lastly, since the NP-DAAO system is designed for cancer therapy, we proved its efficacy in killing SKOV-3, U87 and HCT-116 cancer cells.
      247Scopus© Citations 15