Rice, James H.

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Rice, James H.
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Rice, James H.

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  • Publication
    Nanoscale optical imaging by atomic force infrared microscopy
    (RSC Publishing, 2010-05-01)
    Rice, James H. 
    This review outlines progress in atomic force infrared microscopy, reviewing the methodology and its application in nanoscale infrared absorption imaging of both biological and functional materials, including an outline of where this emerging method has been applied to image cellular systems in aqueous environments.
      842Scopus© Citations 30
  • Publication
    Sub-wavelength infrared imaging of lipids
    (Optical Society of America, 2011-01)
    Yarrow, Fiona 
    ;
    Kennedy, Eamonn 
    ;
    Salaun, Frederic 
    ;
    Rice, James H. 
    Infrared absorption spectroscopy of lipid layers was performed by combining optics and scanning probe microscopy. This experimental approach enables sub-diffraction IR imaging with a spatial resolution on the nanometer scale of 1, 2-dioleoyl-sn-glycero-3-phosphocholine lipid layers.
      346Scopus© Citations 18
  • Publication
    Beyond the diffraction limit : far-field fluorescence imaging with ultrahigh resolution
    (RSC Publishing, 2007-11)
    Rice, James H. 
    Fluorescence microscopy is an important and extensively utilised tool for imaging biological systems. However, the image resolution that can be obtained has a limit as defined through the laws of diffraction. Demand for improved resolution has stimulated research into developing methods to image beyond the diffraction limit based on far-field fluorescence microscopy techniques. Rapid progress is being made in this area of science with methods emerging that enable fluorescence imaging in the far-field to possess a resolution well beyond the diffraction limit. This review outlines developments in far-field fluorescence methods which enable ultrahigh resolution imaging and application of these techniques to biology. Future possible trends and directions in far-field fluorescence imaging with ultrahigh resolution are also outlined.
      861Scopus© Citations 27
  • Publication
    Localized IR spectroscopy of hemoglobin
    (Springer, 2011-02)
    Yarrow, Fiona 
    ;
    Rice, James H. 
    IR absorption spectroscopy of hemoglobin was performed using an infrared (IR) optical parametric oscillator laser and a commercial atomic force microscope (AFM) in a novel experimental arrangement based on the use of a bottom-up excitation alignment. This experimental approach enables detection of protein samples with resolution much higher than that of standard IR spectroscopy. Presented here are AFM-based IR absorption spectra of micron-sized hemoglobin features.
      538Scopus© Citations 5
  • Publication
    Nanoscale infrared absorption imaging permits non-destructive intracellular photosensitizer localization for subcellular uptake analysis
    (RSC Publishing, 2013-06-07)
    Kennedy, Eamonn 
    ;
    Al-Majmaie, Rasoul 
    ;
    Al-Rubeai, Mohamed 
    ;
    Zerulla, Dominic 
    ;
    Rice, James H. 
    The most immediate biological and medical advantages of therapeutic agent localization on the nanoscale arise from the increased understanding of targeted delivery, selectivity and intracellular distribution that are gained by imaging at the resolution scale of individual nanovectors and therapeutic agents themselves. This paper reports on the use of a nanoscale resolution chemical imaging method, infrared (IR) nanospectral absorption imaging, used to map the subcellular localization of a photoactive therapeutic agent - toluidine blue-conjugated gold nanoparticles (TBO) within nanoscale subsections of single colon adenocarcinoma cells. By comparison of photosensitizer distribution with diffraction limited optical imaging, the benefits of IR nanospectral localization are highlighted and the spatial and spectral accuracy of the non-destructive IR imaging method is confirmed. IR spectral ratio imaging is presented as a means to map intracellular nanoparticle density at sub 50 nm lateral resolution with IR nanospectroscopy enabling distinction of nanoparticle seeded cells from a control group with 95% confidence. In this way we illustrate that IR absorption nanoimaging combined with IR point source data does not only yield intracellular drug detection on the order of nanometres, but also permits extension of the AFM-IR technique from subcellular analysis up to studies of cell numbers that are statistically significant.
      534Scopus© Citations 30