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  5. Extraction and yield optimisation of fucose, glucans and associated antioxidant activities from laminaria digitata by applying response surface methodology to high intensity ultrasound-assisted extraction
 
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Extraction and yield optimisation of fucose, glucans and associated antioxidant activities from laminaria digitata by applying response surface methodology to high intensity ultrasound-assisted extraction

File(s)
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Download marinedrugs-16-00257-v2.pdf1.3 MB
Author(s)
García-Vaquero, Marco 
Rajauria, Gaurav 
Tiwari, Brijesh K. 
Sweeney, Torres 
O'Doherty, John V. 
Uri
http://hdl.handle.net/10197/11847
Date Issued
30 July 2018
Date Available
18T16:33:22Z January 2021
Abstract
The objectives of this study were to employ response surface methodology (RSM) to investigate and optimize the effect of ultrasound-assisted extraction (UAE) variables, temperature, time and amplitude on the yields of polysaccharides (fucose and total glucans) and antioxidant activities (ferric reducing antioxidant power (FRAP) and 1,1-diphenyl-2-picryl-hydrazyl radical scavenging activity (DPPH)) from Laminaria digitata, and to explore the suitability of applying the optimum UAE conditions for L. digitata to other brown macroalgae (L. hyperborea and Ascophyllum nodosum). The RSM with three-factor, four-level Box-Behnken Design (BBD) was used to study and optimize the extraction variables. A second order polynomial model fitted well to the experimental data with R2 values of 0.79, 0.66, 0.64, 0.73 for fucose, total glucans, FRAP and DPPH, respectively. The UAE parameters studied had a significant influence on the levels of fucose, FRAP and DPPH. The optimised UAE conditions (temperature = 76 ◦C, time = 10 min and amplitude = 100%) achieved yields of fucose (1060.7 ± 70.6 mg/100 g dried seaweed (ds)), total glucans (968.6 ± 13.3 mg/100 g ds), FRAP (8.7 ± 0.5 µM trolox/mg freeze-dried extract (fde)) and DPPH (11.0 ± 0.2%) in L. digitata. Polysaccharide rich extracts were also attained from L. hyperborea and A. nodosum with variable results when utilizing the optimum UAE conditions for L. digitata.
Sponsorship
Science Foundation Ireland
Type of Material
Journal Article
Publisher
MDPI
Journal
Marine Drugs
Volume
16
Issue
8
Copyright (Published Version)
2018 the Authors
Keywords
  • Laminaria

  • Seaweed

  • Phenols

  • Glucans

  • Fucose

  • Polysaccharides

  • Free radical scavenge...

  • Chemical fractionatio...

  • Ultrasonic waves

DOI
10.3390/md16080257
Language
English
Status of Item
Peer reviewed
ISSN
1660-3397
This item is made available under a Creative Commons License
https://creativecommons.org/licenses/by-nc-nd/3.0/ie/
Owning collection
Agriculture and Food Science Research Collection
Scopus© citations
52
Acquisition Date
Feb 3, 2023
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Views
519
Acquisition Date
Feb 4, 2023
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Downloads
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Acquisition Date
Feb 4, 2023
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