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  5. Comparison of three methods for the detection of Angiostrongylus vasorum in the final host
 
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Comparison of three methods for the detection of Angiostrongylus vasorum in the final host

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Author(s)
Houpin, E. 
McCarthy, Grainne 
Ferrand, M. 
De Waal, Theo 
O'Neill, Emma J. 
Zintl, Annetta 
Uri
http://hdl.handle.net/10197/8082
Date Issued
15 April 2016
Date Available
15T01:00:13Z April 2017
Abstract
Angiostrongylosis is potentially fatal parasitic nematode infection affecting dogs which can be difficult to diagnose. In recent years several microscopical, serological and molecular detection methods have been developed, however there are few studies that have compared the relative performance of these methods. Screening necropsy material from an opportunistic sample of 140 foxes (82 of which were considered to be infected with Angiostrongylus vasorum), indicated sensitivities of 84.1% for dissection and visual examination of plucks, 69.5% for nested PCR of an 18S rRNA fragment and 76.8% for a canine A. vasorum antigen detection test (IDEXX Angio Detect) of tissue fluid samples respectively. Agreement between the tests ranged from 45.6 to 79.7%. A novel nested PCR-RFLP for the detection and identification of canid lungworm spp. is described.
Type of Material
Journal Article
Publisher
Elsevier
Journal
Veterinary Parasitology
Volume
220
Start Page
54
End Page
58
Copyright (Published Version)
2016 Elsevier
Keywords
  • Angiostrongylus vasor...

  • Crenosoma vulpis

  • Vulpes vulpes

  • Diagnosis

  • Post-mortem examinati...

  • IDEXX Angio Detect ki...

  • Nested PCR-RFLP

DOI
10.1016/j.vetpar.2016.02.023
Language
English
Status of Item
Peer reviewed
This item is made available under a Creative Commons License
https://creativecommons.org/licenses/by-nc-nd/3.0/ie/
Owning collection
Veterinary Medicine Research Collection
Scopus© citations
16
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Feb 5, 2023
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