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  5. First confirmation by PCR of Jaagsiekte sheep retrovirus in Ireland and prevalence of ovine pulmonary adenocarcinoma in adult sheep at slaughter
 
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First confirmation by PCR of Jaagsiekte sheep retrovirus in Ireland and prevalence of ovine pulmonary adenocarcinoma in adult sheep at slaughter

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Author(s)
Lee, Alison Marie 
Wolfe, Alan 
Cassidy, Joseph P. 
Messam, Locksley L. McV. 
et al. 
Uri
http://hdl.handle.net/10197/10354
Date Issued
19 December 2017
Date Available
08T13:28:24Z May 2019
Abstract
Background: Ovine pulmonary adenocarcinoma (OPA), caused by Jaagsiekte sheep retrovirus (JSRV), is characterised by the development of invariably fatal lung tumours primarily in adult sheep. High infection rates and disease prevalence can develop during initial infection of flocks, leading to on-farm economic losses and animal welfare issues in sheep with advanced disease. The disease has been reported in Ireland and is notifiable, but the presence of JSRV has never been confirmed using molecular methods in this country. Additionally, due to the difficulties in ante-mortem diagnosis (especially of latently-infected animals, or those in the very early stages of disease), accurate information regarding national prevalence and distribution is unavailable. This study aimed to confirm the presence of JSRV in Ireland and to obtain estimates regarding prevalence and distribution by means of an abattoir survey utilising gross examination, histopathology, JSRV-specific reverse transcriptase polymerase chain reaction (RT-PCR) and SU protein specific immunohistochemistry (IHC) to examine the lungs of adult sheep. Results: Lungs from 1911 adult sheep were examined macroscopically in the abattoir and 369 were removed for further testing due to the presence of gross lesions of any kind. All 369 were subject to histopathology and RT-PCR, and 46 to IHC. Thirty-one lungs (31/1911, 1.6%) were positive for JSRV by RT-PCR and/or IHC but only ten cases of OPA were confirmed (10/1911, 0.5%) Four lung tumours not associated with JSRV were also identified. JSRV-positive sheep tended to cluster within the same flocks, and JSRV-positive sheep were identified in the counties of Donegal, Kerry, Kilkenny, Offaly, Tipperary, Waterford and Wicklow. Conclusions: The presence of JSRV has been confirmed in the Republic of Ireland for the first time using molecular methods (PCR) and IHC. In addition, an estimate of OPA prevalence in sheep at slaughter and information regarding distribution of JSRV infection has been obtained. The prevalence estimate appears similar to that of the United Kingdom (UK). Results also indicate that the virus has a diverse geographical distribution throughout Ireland. These data highlights the need for further research to establish national control and monitoring strategies.
Other Sponsorship
Scottish Government Rural and Environment Science
Analytical Services Division (RESAS)
Type of Material
Journal Article
Publisher
BioMed Central
Journal
Irish Veterinary Journal
Volume
70
Issue
33
Copyright (Published Version)
2017 the Authors
Keywords
  • Jaagsiekte

  • Jsrv

  • Jaagsiekte sheep retr...

  • Opa

  • Ovine pulmonary adeno...

  • Diagnostics

  • Neoplasia

  • Retrovirus

  • Prevalence

  • Ireland

DOI
10.1186/s13620-017-0111-z
Language
English
Status of Item
Peer reviewed
ISSN
0368-0762
This item is made available under a Creative Commons License
https://creativecommons.org/licenses/by-nc-nd/3.0/ie/
Owning collection
Veterinary Medicine Research Collection
Scopus© citations
12
Acquisition Date
Feb 5, 2023
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