Options
Surface functionalization-dependent localization and affinity of SiO2 nanoparticles within the biofilm EPS matrix
Date Issued
2020-12
Date Available
2021-07-01T15:40:04Z
Abstract
The contribution of the biofilm extracellular polymeric substance (EPS) matrix to reduced antimicrobial sus-ceptibility in biofilms is widely recognised. As such, the direct targeting of the EPS matrix is a promising biofilmcontrol strategy that allows for the disruption of the matrix, thereby allowing a subsequent increase in suscep-tibility to antimicrobial agents. To this end, surface-functionalized nanoparticles (NPs) have received considerableattention. However, the fundamental understanding of the interactions occurring between engineered NPs andthe biofilm EPS matrix has not yet been fully elucidated. An insight into the underlying mechanisms involvedwhen a NP interacts with the EPS matrix will aid in the design of more efficient NPs for biofilm control. Here wedemonstrate the use of highly specificfluorescent probes in confocal laser scanning microscopy (CLSM) toillustrate the distribution of EPS macromolecules within the biofilm. Thereafter, a three-dimensional (3D)colocalization analysis was used to assess the affinity of differently functionalized silica NPs (SiNPs) and EPSmacromolecules fromPseudomonasfluorescensbiofilms. Results show that both the charge and surface functionalgroups of SiNPs dramatically affected the extent to which SiNPs interacted and localized with EPS macromole-cules, including proteins, polysaccharides and DNA. Hypotheses are also presented about the possible physico-chemical interactions which may be dominant in EPS matrix-NP interactions. This research not only develops aninnovative CLSM-based methodology for elucidating biofilm-nanoparticle interactions but also provides a plat-form on which to build more efficient NP systems for biofilm control.
Sponsorship
Science Foundation Ireland
Type of Material
Journal Article
Publisher
Elsevier
Journal
Biofilm
Volume
2
Copyright (Published Version)
2020 the Authors
Language
English
Status of Item
Peer reviewed
ISSN
2590-2075
This item is made available under a Creative Commons License
File(s)
Owning collection
Scopus© citations
20
Acquisition Date
Mar 28, 2024
Mar 28, 2024
Views
547
Last Week
1
1
Last Month
1
1
Acquisition Date
Mar 28, 2024
Mar 28, 2024
Downloads
265
Last Week
2
2
Last Month
8
8
Acquisition Date
Mar 28, 2024
Mar 28, 2024