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  5. Nanoparticle accumulation and transcytosis in brain endothelial cell layers
 
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Nanoparticle accumulation and transcytosis in brain endothelial cell layers

Author(s)
Ye, Dong  
Nic Raghnaill, Michelle  
Bramini, Mattia  
Mahon, Eugene  
Åberg, Christoffer  
Salvati, Anna  
Dawson, Kenneth A.  
Uri
http://hdl.handle.net/10197/5140
Date Issued
2013-09
Date Available
2014-09-01T03:00:07Z
Abstract
The blood–brain barrier (BBB) is a selective barrier, which controls and limits access to the central nervous system (CNS). The selectivity of the BBB relies on specialized characteristics of the endothelial cells that line the microvasculature, including the expression of intercellular tight junctions, which limit paracellular permeability. Several reports suggest that nanoparticles have a unique capacity to cross the BBB. However, direct evidence of nanoparticle transcytosis is difficult to obtain, and we found that typical transport studies present several limitations when applied to nanoparticles. In order to investigate the capacity of nanoparticles to access and transport across the BBB, several different nanomaterials, including silica, titania and albumin- or transferrin-conjugated gold nanoparticles of different sizes, were exposed to a human in vitro BBB model of endothelial hCMEC/D3 cells. Extensive transmission electron microscopy imaging was applied in order to describe nanoparticle endocytosis and typical intracellular localisation, as well as to look for evidence of eventual transcytosis. Our results show that all of the nanoparticles were internalised, to different extents, by the BBB model and accumulated along the endo–lysosomal pathway. Rare events suggestive of nanoparticle transcytosis were also observed for several of the tested materials.
Sponsorship
Science Foundation Ireland
Other Sponsorship
INSPIRE (Integrated NanoScience Platform for Ireland)
EU FP7 Small Collaborative projects NeuroNano
NanoTransKinetics
Type of Material
Journal Article
Publisher
Royal Society of Chemistry
Journal
Nanoscale
Volume
5
Issue
22
Start Page
11153
End Page
11165
Copyright (Published Version)
2013 The Royal Society of Chemistry
Subjects

Blood-brain barrier

Transport studies

Nanomaterials

DOI
10.1039/C3NR02905K
Language
English
Status of Item
Peer reviewed
ISSN
2040-3364
This item is made available under a Creative Commons License
https://creativecommons.org/licenses/by-nc-nd/3.0/ie/
File(s)
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Ye_et_al_manuscript_revised.pdf

Size

2.96 MB

Format

Adobe PDF

Checksum (MD5)

dfb2838a28b58ff5bc4ed479904d1249

Owning collection
Chemistry Research Collection

Item descriptive metadata is released under a CC-0 (public domain) license: https://creativecommons.org/public-domain/cc0/.
All other content is subject to copyright.

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