Biosynthesis of amphotericin derivatives lacking exocyclic carboxyl groups
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|Title:||Biosynthesis of amphotericin derivatives lacking exocyclic carboxyl groups||Authors:||Carmody, Maria
Rai, Dilip K.
|Permanent link:||http://hdl.handle.net/10197/10038||Date:||3-Aug-2005||Online since:||2019-04-18T09:23:31Z||Abstract:||Amphotericin B is a medically important antifungal antibiotic that is also active against human immunodeficiency virus, Leishmania parasites, and prion diseases. The therapeutic use of amphotericin B is restricted by severe side effects that can be moderated by liposomal formulation or structural alteration. Chemical modification has shown that suppression of charge on the exocyclic carboxyl group of amphotericin B substantially reduces toxicity. We report targeted deletions of the amphN cytochrome P450 gene from the chromosome of the amphotericin-producing bacterium Streptomyces nodosus. The mutant strains produced amphotericin analogues in which methyl groups replace the exocyclic carboxyl groups. These compounds retained antifungal activity and had reduced hemolytic activity.||Funding Details:||Higher Education Authority||Type of material:||Journal Article||Publisher:||ASBMB||Journal:||Journal of Biological Chemistry||Volume:||280||Issue:||41||Start page:||34420||End page:||34426||Copyright (published version):||2005 The American Society for Biochemistry and Molecular Biology||Keywords:||Streptomyce; Escherichia coli; Bacteriophages; Carbon; Amphotericin B; Polyenes||DOI:||10.1074/jbc.M506689200||Other versions:||http://www.jbc.org/content/280/41/34420.full||Language:||en||Status of Item:||Peer reviewed|
|Appears in Collections:||Biomolecular and Biomedical Science Research Collection|
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