Biosynthesis of amphotericin derivatives lacking exocyclic carboxyl groups

DC FieldValueLanguage
dc.contributor.authorCarmody, Maria-
dc.contributor.authorMurphy, Barry-
dc.contributor.authorByrne, Barry-
dc.contributor.authorPower, Patrick-
dc.contributor.authorRai, Dilip K.-
dc.contributor.authorRawlings, Bernard-
dc.contributor.authorCaffrey, Patrick-
dc.date.accessioned2019-04-18T09:23:31Z-
dc.date.available2019-04-18T09:23:31Z-
dc.date.copyright2005 The American Society for Biochemistry and Molecular Biologyen_US
dc.date.issued2005-08-03-
dc.identifier.citationJournal of Biological Chemistryen_US
dc.identifier.issn0021-9258-
dc.identifier.urihttp://hdl.handle.net/10197/10038-
dc.description.abstractAmphotericin B is a medically important antifungal antibiotic that is also active against human immunodeficiency virus, Leishmania parasites, and prion diseases. The therapeutic use of amphotericin B is restricted by severe side effects that can be moderated by liposomal formulation or structural alteration. Chemical modification has shown that suppression of charge on the exocyclic carboxyl group of amphotericin B substantially reduces toxicity. We report targeted deletions of the amphN cytochrome P450 gene from the chromosome of the amphotericin-producing bacterium Streptomyces nodosus. The mutant strains produced amphotericin analogues in which methyl groups replace the exocyclic carboxyl groups. These compounds retained antifungal activity and had reduced hemolytic activity.en_US
dc.description.sponsorshipHigher Education Authorityen_US
dc.format.mediumPrint-Electronic-
dc.language.isoenen_US
dc.publisherASBMBen_US
dc.rightsThis is the author's version of an article that has been published in the Journal of Biological Chemistry. The final published version is available at the following DOI: 10.1074/jbc.M506689200.en_US
dc.subjectStreptomyceen_US
dc.subjectEscherichia colien_US
dc.subjectBacteriophagesen_US
dc.subjectCarbonen_US
dc.subjectAmphotericin Ben_US
dc.subjectPolyenesen_US
dc.titleBiosynthesis of amphotericin derivatives lacking exocyclic carboxyl groupsen_US
dc.title.alternativeBiosynthesis of less toxic amphotericinsen_US
dc.typeJournal Articleen_US
dc.internal.authorcontactotherpatrick.caffrey@ucd.ieen_US
dc.internal.webversionshttp://www.jbc.org/content/280/41/34420.full-
dc.statusPeer revieweden_US
dc.identifier.volume280en_US
dc.identifier.issue41en_US
dc.identifier.startpage34420en_US
dc.identifier.endpage34426en_US
dc.identifier.doi10.1074/jbc.M506689200-
dc.neeo.contributorCarmody|Maria|aut|-
dc.neeo.contributorMurphy|Barry|aut|-
dc.neeo.contributorByrne|Barry|aut|-
dc.neeo.contributorPower|Patrick|aut|-
dc.neeo.contributorRai|Dilip K.|aut|-
dc.neeo.contributorRawlings|Bernard|aut|-
dc.neeo.contributorCaffrey|Patrick|aut|-
dc.description.othersponsorshipEuropean Unionen_US
dc.date.updated2018-07-05T08:24:29Z-
dc.identifier.grantidGENOVA QLRT-1999-00095-
item.fulltextWith Fulltext-
item.grantfulltextopen-
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