Mutations in gamma adducin are associated with inherited cerebral palsy

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Title: Mutations in gamma adducin are associated with inherited cerebral palsy
Authors: Kruer, Michael C.
Jepperson, Tyler
Dutta, Sudeshna
Mooney, Catherine
et al.
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Date: 9-Jul-2013
Online since: 2019-04-23T09:52:06Z
Abstract: Objective: Cerebral palsy is estimated to affect nearly 1 in 500 children, and although prenatal and perinatal contributors have been well characterized, at least 20% of cases are believed to be inherited. Previous studies have identified mutations in the actin‐capping protein KANK1 and the adaptor protein‐4 complex in forms of inherited cerebral palsy, suggesting a role for components of the dynamic cytoskeleton in the genesis of the disease. Methods: We studied a multiplex consanguineous Jordanian family by homozygosity mapping and exome sequencing, then used patient‐derived fibroblasts to examine functional consequences of the mutation we identified in vitro. We subsequently studied the effects of adducin loss of function in Drosophila. Results: We identified a homozygous c.1100G>A (p.G367D) mutation in ADD3, encoding gamma adducin in all affected members of the index family. Follow‐up experiments in patient fibroblasts found that the p.G367D mutation, which occurs within the putative oligomerization critical region, impairs the ability of gamma adducin to associate with the alpha subunit. This mutation impairs the normal actin‐capping function of adducin, leading to both abnormal proliferation and migration in cultured patient fibroblasts. Loss of function studies of the Drosophila adducin ortholog hts confirmed a critical role for adducin in locomotion. Interpretation: Although likely a rare cause of cerebral palsy, our findings indicate a critical role for adducins in regulating the activity of the actin cytoskeleton, suggesting that impaired adducin function may lead to neuromotor impairment and further implicating abnormalities of the dynamic cytoskeleton as a pathogenic mechanism contributing to cerebral palsy.
Funding Details: Wellcome Trust
Type of material: Journal Article
Publisher: Wiley
Journal: Annals of Neurology
Volume: 74
Issue: 6
Start page: 805
End page: 814
Copyright (published version): 2014 the Authors
Keywords: Cerebral palsyKANK1Adaptor protein‐4 complexInherited cerebral palsyp.G367D mutationAdducins
DOI: 10.1002/ana.23971
Language: en
Status of Item: Peer reviewed
Appears in Collections:Computer Science Research Collection

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