Investigation of facilitative urea transporters in the human gastrointestinal tract

DC FieldValueLanguage
dc.contributor.authorWalpole, Caragh-
dc.contributor.authorMcGrane, Alison-
dc.contributor.authorAl-mousawi, Hashemeya-
dc.contributor.authorWinter, Desmond C.-
dc.contributor.authorBaird, Alan W.-
dc.contributor.authorStewart, Gavin-
dc.date.accessioned2019-04-24T10:35:21Z-
dc.date.available2019-04-24T10:35:21Z-
dc.date.copyright2018 the Authorsen_US
dc.date.issued2018-08-12-
dc.identifier.citationPhysiological Reportsen_US
dc.identifier.issn2051-817X-
dc.identifier.urihttp://hdl.handle.net/10197/10116-
dc.description.abstractThe symbiotic relationship between humans and their intestinal microbiomeis supported by urea nitrogen salvaging. Previous studies have shown thatcolonic UT-B urea transporters play a significant role in this important physi-ological process. This current study investigated UT-A and UT-B urea trans-porter expression along the human gastrointestinal tract. Initial end-pointPCR experiments determined that UT-A RNA was predominantly expressedin the small intestine, while UT-B RNA was expressed in stomach, small intes-tine, and colon. Using western blotting experiments, a strong 40–60 kDa UT-B signal was found to be abundant in both ileum and colon. Importantly, thissignal was deglycosylated by PNGaseF enzyme treatment to a core protein of30 kDa in both tissues. Further immunolocalization studies revealed UT-Btransporter proteins were present at the apical membrane of the villi in theileum, but predominantly at the basolateral membrane of the colonic surfaceepithelial cells. Finally, a blind scoring immunolocalization study suggestedthat there was no significant difference in UT-B abundance throughout thecolon (NS, ANOVA,N=5–21). In conclusion, this current study suggestedUT-B to be the main human intestinal urea transporter. Intriguingly, thesedata suggested that the same UT-B isoform was present in all intestinalepithelial cells, but that the precise cellular location varied.en_US
dc.description.sponsorshipScience Foundation Irelanden_US
dc.language.isoenen_US
dc.publisherWileyen_US
dc.rightsPhysiological Reports published by Wiley Periodicals, Inc. on behalf of The Physiological Society and the American Physiological Society. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.en_US
dc.subjectGastrointestinal tracten_US
dc.subjectHumanen_US
dc.subjectUT‐Aen_US
dc.subjectUT‐Ben_US
dc.titleInvestigation of facilitative urea transporters in the human gastrointestinal tracten_US
dc.typeJournal Articleen_US
dc.internal.authorcontactothergavin.stewart@ucd.ieen_US
dc.statusPeer revieweden_US
dc.identifier.volume6en_US
dc.identifier.issue15en_US
dc.identifier.startpagee13826en_US
dc.identifier.doi10.14814/phy2.13826-
dc.neeo.contributorWalpole|Caragh|aut|-
dc.neeo.contributorMcGrane|Alison|aut|-
dc.neeo.contributorAl-mousawi|Hashemeya|aut|-
dc.neeo.contributorWinter|Desmond C.|aut|-
dc.neeo.contributorBaird|Alan W.|aut|-
dc.neeo.contributorStewart|Gavin|aut|-
dc.date.updated2018-08-15T16:51:03Z-
dc.identifier.grantid11/RFP.1/BMT/3088-
item.fulltextWith Fulltext-
item.grantfulltextopen-
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Biology & Environmental Science Research Collection
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