Peptigram: a web-based application for peptidomics data visualization

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Title: Peptigram: a web-based application for peptidomics data visualization
Authors: Manguy, Jean
Jehl, Peter
Dillon, Eugène T.
Davey, Norman E.
Shields, Denis C.
Holton, Thérèse A.
Permanent link: http://hdl.handle.net/10197/10155
Date: 2-Dec-2016
Online since: 2019-04-25T09:08:30Z
Abstract: Tandem mass spectrometry (MS/MS) techniques, developed for protein identification, are increasingly being applied in the field of peptidomics. Using this approach, the set of protein fragments observed in a sample of interest can be determined to gain insights into important biological processes such as signaling and other bioactivities. As the peptidomics era progresses, there is a need for robust and convenient methods to inspect and analyze MS/MS derived data. Here, we present Peptigram, a novel tool dedicated to the visualization and comparison of peptides detected by MS/MS. The principal advantage of Peptigram is that it provides visualizations at both the protein and peptide level, allowing users to simultaneously visualize the peptide distributions of one or more samples of interest, mapped to their parent proteins. In this way rapid comparisons between samples can be made in terms of their peptide coverage and abundance. Moreover, Peptigram integrates and displays key sequence features from external databases and links with peptide analysis tools to offer the user a comprehensive peptide discovery resource. Here, we illustrate the use of Peptigram on a data set of milk hydrolysates. For convenience, Peptigram is implemented as a web application, and is freely available for academic use at http://bioware.ucd.ie/peptigram.
Funding Details: Enterprise Ireland
Irish Research Council
Science Foundation Ireland
Type of material: Journal Article
Publisher: ACS Publications
Journal: Journal of Proteome Research
Volume: 16
Issue: 2
Start page: 712
End page: 719
Copyright (published version): 2016 American Chemical Society
Keywords: Data visualisationPeptidesPeptidomicsProteomicsMass spectrometrySample comparisonProteolysis
DOI: 10.1021/acs.jproteome.6b00751
Other versions: https://www.ncbi.nlm.nih.gov/pubmed/?term=peptigram
https://pubs.acs.org/doi/10.1021/acs.jproteome.6b00751
Language: en
Status of Item: Peer reviewed
Appears in Collections:Conway Institute Research Collection
Biomolecular and Biomedical Science Research Collection
Medicine Research Collection

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