Synthesis, characterization and programmable toxicity of iron oxide nanoparticles conjugated with D-amino acid oxidase

Title: Synthesis, characterization and programmable toxicity of iron oxide nanoparticles conjugated with D-amino acid oxidase
Authors: Balzaretti, Riccardo
Meder, Fabian
Monopoli, Marco P.
Boselli, Luca
et al.
Permanent link: http://hdl.handle.net/10197/10275
Date: 5-Jan-2017
Online since: 2019-05-02T09:38:06Z
Abstract: D-amino acid oxidase (DAAO) is an enzyme which generates reactive oxygen species (ROS) and it is believed to have potential uses as a novel therapeutic molecule if internalized by cancer cells or if they are localized close to their plasma membrane. When conjugated onto iron oxide nanoparticles (NPs), the enzyme can be magnetically directed to targeted locations with an increased efficacy. A subsequent injection of DAAO substrate D-alanine can initiate ROS production and induce apoptosis of cells surrounding the NP-DAAO complex. Here, we describe a platform for optimal bioconjugation using monodisperse γ-Fe2O3 NPs (∼10 nm) resulting in high DAAO loading, stable NP-DAAO dispersions and more than 90% enzymatic activity recovery, which is retained using the particles in human serum. Lastly, since the NP-DAAO system is designed for cancer therapy, we proved its efficacy in killing SKOV-3, U87 and HCT-116 cancer cells.
Funding Details: European Commission Horizon 2020
Science Foundation Ireland
Type of material: Journal Article
Publisher: Royal Society of Chemistry
Journal: RSC Advances
Volume: 7
Issue: 3
Start page: 1439
End page: 1442
Copyright (published version): 2017 The Royal Society of Chemistry
Keywords: Reactive oxygen species (ROS)Cancer cellsNanoparticles (NPs)γ-Fe2O3D-amino acid oxidase (DAAO)Cancer therapy
DOI: 10.1039/c6ra25349k
Language: en
Status of Item: Peer reviewed
Appears in Collections:Centre for Bionano Interactions (CBNI) Research Collection

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