Synthesis, characterization and programmable toxicity of iron oxide nanoparticles conjugated with D-amino acid oxidase
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|Title:||Synthesis, characterization and programmable toxicity of iron oxide nanoparticles conjugated with D-amino acid oxidase||Authors:||Balzaretti, Riccardo
Monopoli, Marco P.
|Permanent link:||http://hdl.handle.net/10197/10275||Date:||5-Jan-2017||Online since:||2019-05-02T09:38:06Z||Abstract:||D-amino acid oxidase (DAAO) is an enzyme which generates reactive oxygen species (ROS) and it is believed to have potential uses as a novel therapeutic molecule if internalized by cancer cells or if they are localized close to their plasma membrane. When conjugated onto iron oxide nanoparticles (NPs), the enzyme can be magnetically directed to targeted locations with an increased efficacy. A subsequent injection of DAAO substrate D-alanine can initiate ROS production and induce apoptosis of cells surrounding the NP-DAAO complex. Here, we describe a platform for optimal bioconjugation using monodisperse γ-Fe2O3 NPs (∼10 nm) resulting in high DAAO loading, stable NP-DAAO dispersions and more than 90% enzymatic activity recovery, which is retained using the particles in human serum. Lastly, since the NP-DAAO system is designed for cancer therapy, we proved its efficacy in killing SKOV-3, U87 and HCT-116 cancer cells.||Funding Details:||European Commission Horizon 2020
Science Foundation Ireland
|Type of material:||Journal Article||Publisher:||Royal Society of Chemistry||Journal:||RSC Advances||Volume:||7||Issue:||3||Start page:||1439||End page:||1442||Copyright (published version):||2017 The Royal Society of Chemistry||Keywords:||Reactive oxygen species (ROS); Cancer cells; Nanoparticles (NPs); γ-Fe2O3; D-amino acid oxidase (DAAO); Cancer therapy||DOI:||10.1039/c6ra25349k||Language:||en||Status of Item:||Peer reviewed|
|Appears in Collections:||Centre for Bionano Interactions (CBNI) Research Collection|
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