Options
Synthesis, characterization and programmable toxicity of iron oxide nanoparticles conjugated with D-amino acid oxidase
Date Issued
2017-01-05
Date Available
2019-05-02T09:38:06Z
Abstract
D-amino acid oxidase (DAAO) is an enzyme which generates reactive oxygen species (ROS) and it is believed to have potential uses as a novel therapeutic molecule if internalized by cancer cells or if they are localized close to their plasma membrane. When conjugated onto iron oxide nanoparticles (NPs), the enzyme can be magnetically directed to targeted locations with an increased efficacy. A subsequent injection of DAAO substrate D-alanine can initiate ROS production and induce apoptosis of cells surrounding the NP-DAAO complex. Here, we describe a platform for optimal bioconjugation using monodisperse γ-Fe2O3 NPs (∼10 nm) resulting in high DAAO loading, stable NP-DAAO dispersions and more than 90% enzymatic activity recovery, which is retained using the particles in human serum. Lastly, since the NP-DAAO system is designed for cancer therapy, we proved its efficacy in killing SKOV-3, U87 and HCT-116 cancer cells.
Sponsorship
European Commission Horizon 2020
Science Foundation Ireland
Other Sponsorship
German Research Foundation (DFG)
Type of Material
Journal Article
Publisher
Royal Society of Chemistry
Journal
RSC Advances
Volume
7
Issue
3
Start Page
1439
End Page
1442
Copyright (Published Version)
2017 The Royal Society of Chemistry
Language
English
Status of Item
Peer reviewed
This item is made available under a Creative Commons License
File(s)
No Thumbnail Available
Name
Synthesis, characterization and programmable toxicity of iron oxide nanoparticles conjugated with D-amino acid oxidase.pdf
Size
541.76 KB
Format
Owning collection
Scopus© citations
14
Acquisition Date
Mar 28, 2024
Mar 28, 2024
Views
728
Acquisition Date
Mar 28, 2024
Mar 28, 2024
Downloads
243
Last Month
7
7
Acquisition Date
Mar 28, 2024
Mar 28, 2024