Graphene Nanoflake Uptake Mediated by Scavenger Receptors

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Title: Graphene Nanoflake Uptake Mediated by Scavenger Receptors
Authors: Alnasser, Fatima
Castagnola, Valentina
Boselli, Luca
Esquivel-Gaona, Margarita
Dawson, Kenneth A.
et al.
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Date: 10-Jan-2019
Online since: 2019-05-02T10:18:34Z
Abstract: The biological interactions of graphene have been extensively investigated over the last 10 years. However, very little is known about graphene interactions with the cell surface and how the graphene internalization process is driven and mediated by specific recognition sites at the interface with the cell. In this work, we propose a methodology to investigate direct molecular correlations between the biomolecular corona of graphene and specific cell receptors, showing that key protein recognition motifs, presented on the nanomaterial surface, can engage selectively with specific cell receptors. We consider the case of apolipoprotein A-I, found to be very abundant in the graphene protein corona, and observe that the uptake of graphene nanoflakes is somewhat increased in cells with greatly elevated expression of scavenger receptors B1, suggesting a possible mechanism of endogenous interaction. The uptake results, obtained by flow cytometry, have been confirmed using Raman microspectroscopic mapping, exploiting the strong Raman signature of graphene.
Funding Details: European Commission Horizon 2020
European Commission - Seventh Framework Programme (FP7)
Irish Research Council
Science Foundation Ireland
Type of material: Journal Article
Publisher: ACS Publications
Journal: Nano Letters
Volume: 19
Issue: 2
Start page: 1260
End page: 1268
Copyright (published version): 2019 American Chemical Society
Keywords: GrapheneProteinCoronaScavenger receptorsNanobio interactions
DOI: 10.1021/acs.nanolett.8b04820
Language: en
Status of Item: Peer reviewed
Appears in Collections:Centre for Bionano Interactions (CBNI) Research Collection

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