Characterization of carboxylate nanoparticle adhesion with the fungal pathogen Candida albicans

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Title: Characterization of carboxylate nanoparticle adhesion with the fungal pathogen Candida albicans
Authors: Lyden, Amy
Lombardi, Lisa
Sire, Wilfried
Li, Peng
Simpson, Jeremy C.
Butler, Geraldine
Lee, Gil U.
Permanent link: http://hdl.handle.net/10197/10322
Date: 11-Oct-2017
Online since: 2019-05-07T13:48:57Z
Abstract: Candida albicans is the lead fungal pathogen of nosocomial bloodstream infections worldwide and has mortality rates of 43%. Nanoparticles have been identified as a means to improve medical outcomes for Candida infections, enabling sample concentration, serving as contrast agents for in vivo imaging, and delivering therapeutics. However, little is known about how nanoparticles interact with the fungal cell wall. In this report we used laser scanning confocal microscopy to examine the interaction of fluorescent polystyrene nanoparticles of specific surface chemistry and diameter with C. albicans and mutant strains deficient in various C. albicans surface proteins. Carboxylate-functionalized nanoparticles adsorbed mainly to the hyphae of wild-type C. albicans. The dissociative binding constant of the nanoparticles was ∼150, ∼30 and ∼2.5 pM for 40, 100 nm and 200 nm diameter particles, respectively. A significant reduction in particle binding was observed with a Δals3 strain compared to wild-type strains, identifying the Als3 adhesin as the main mediator of this nanoparticle adhesion. In the absence of Als3, nanoparticles bound to germ tubes and yeast cells in a pattern resembling the localization of Als1, indicating Als1 also plays a role. Nanoparticle surface charge was shown to influence binding – positively charged amine-functionalized nanoparticles failed to bind to the hyphal cell wall. Binding of carboxylate-functionalized nanoparticles was observed in the presence of serum, though interactions were reduced. These observations show that Als3 and Als1 are important targets for nanoparticle-mediated diagnostics and therapeutics, and provide direction for optimal diameter and surface characteristics of nanoparticles that bind to the fungal cell wall.
Funding Details: Science Foundation Ireland
Type of material: Journal Article
Publisher: Royal Society of Chemistry
Journal: Nanoscale
Volume: 9
Issue: 41
Start page: 15911
End page: 15922
Copyright (published version): 2017 The Royal Society of Chemistry
Keywords: Candida albicans infectionsInfectious diseasesNosocomial bloodstream infectionsNanotechnologyNanoparticlesBloodstream infections
DOI: 10.1039/c7nr04724j
Language: en
Status of Item: Peer reviewed
Appears in Collections:Conway Institute Research Collection
Biology & Environmental Science Research Collection
Chemistry Research Collection

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