Formulation, Characterization and Stability Assessment of a Food‐Derived Tripeptide, Leucine‐Lysine‐Proline Loaded Chitosan Nanoparticles
|Title:||Formulation, Characterization and Stability Assessment of a Food‐Derived Tripeptide, Leucine‐Lysine‐Proline Loaded Chitosan Nanoparticles||Authors:||Danish, Minna K.
Byrne, Hugh J.
Frías, Jesús M.
Ryan, Sinéad M.
|Permanent link:||http://hdl.handle.net/10197/10332||Date:||Sep-2017||Online since:||2019-05-08T08:07:56Z||Abstract:||The chicken‐ or fish‐derived tripeptide, leucine‐lysine‐proline (LKP), inhibits the angiotensin converting enzyme and may be used as an alternative treatment for prehypertension. However, it has low permeation across the small intestine. The formulation of LKP into a nanoparticle (NP) has the potential to address this issue. LKP‐loaded NPs were produced using an ionotropic gelation technique, using chitosan (CL113). Following optimization of unloaded NPs, a mixture amount design was constructed using variable concentration of CL113 and tripolyphosphate at a fixed LKP concentration. Resultant particle sizes ranged from 120 to 271 nm, zeta potential values from 29 to 37 mV, and polydispersity values from 0.3 to 0.6. A ratio of 6:1 (CL113:TPP) produced the best encapsulation of approximately 65%. Accelerated studies of the loaded NPs indicated stability under normal storage conditions (room temperature). Cytotoxicity assessment showed no significant loss of cell viability and in vitro release studies indicated an initial burst followed by a slower and sustained release.||Funding Details:||Department of Agriculture, Food and the Marine||Type of material:||Journal Article||Publisher:||Wiley||Journal:||Journal of Food Science||Volume:||82||Issue:||9||Start page:||2094||End page:||2104||Copyright (published version):||2017 Institute of Food Technologists||Keywords:||Accelerated thermal stability analysis; ACE inhibition; Chitosan nanoparticles; Food-derived peptide; Mixture amount design||DOI:||10.1111/1750-3841.13824||Language:||en||Status of Item:||Peer reviewed|
|Appears in Collections:||Veterinary Medicine Research Collection|
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