Formulation, Characterization and Stability Assessment of a Food‐Derived Tripeptide, Leucine‐Lysine‐Proline Loaded Chitosan Nanoparticles

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Title: Formulation, Characterization and Stability Assessment of a Food‐Derived Tripeptide, Leucine‐Lysine‐Proline Loaded Chitosan Nanoparticles
Authors: Danish, Minna K.
Vozza, Giuliana
Byrne, Hugh J.
Frías, Jesús M.
Ryan, Sinéad M.
Permanent link: http://hdl.handle.net/10197/10332
Date: Sep-2017
Online since: 2019-05-08T08:07:56Z
Abstract: The chicken‐ or fish‐derived tripeptide, leucine‐lysine‐proline (LKP), inhibits the angiotensin converting enzyme and may be used as an alternative treatment for prehypertension. However, it has low permeation across the small intestine. The formulation of LKP into a nanoparticle (NP) has the potential to address this issue. LKP‐loaded NPs were produced using an ionotropic gelation technique, using chitosan (CL113). Following optimization of unloaded NPs, a mixture amount design was constructed using variable concentration of CL113 and tripolyphosphate at a fixed LKP concentration. Resultant particle sizes ranged from 120 to 271 nm, zeta potential values from 29 to 37 mV, and polydispersity values from 0.3 to 0.6. A ratio of 6:1 (CL113:TPP) produced the best encapsulation of approximately 65%. Accelerated studies of the loaded NPs indicated stability under normal storage conditions (room temperature). Cytotoxicity assessment showed no significant loss of cell viability and in vitro release studies indicated an initial burst followed by a slower and sustained release.
Funding Details: Department of Agriculture, Food and the Marine
Type of material: Journal Article
Publisher: Wiley
Journal: Journal of Food Science
Volume: 82
Issue: 9
Start page: 2094
End page: 2104
Copyright (published version): 2017 Institute of Food Technologists
Keywords: Accelerated thermal stability analysisACE inhibitionChitosan nanoparticlesFood-derived peptideMixture amount design
DOI: 10.1111/1750-3841.13824
Language: en
Status of Item: Peer reviewed
Appears in Collections:Veterinary Medicine Research Collection

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