Comparative study of the structural and physicochemical properties of two food derived antihypertensive tri-peptides, Isoleucine-Proline-Proline and Leucine-Lysine-Proline encapsulated into a chitosan based nanoparticle system

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Title: Comparative study of the structural and physicochemical properties of two food derived antihypertensive tri-peptides, Isoleucine-Proline-Proline and Leucine-Lysine-Proline encapsulated into a chitosan based nanoparticle system
Authors: Danish, Minna K.
Vozza, Giuliana
Byrne, Hugh J.
Frías, Jesús M.
Ryan, Sinéad M.
Permanent link: http://hdl.handle.net/10197/10333
Date: Dec-2017
Online since: 2019-05-08T08:12:57Z
Abstract: Food derived tri-peptides; Leucine-Lysine-Proline (LKP) and Isoleucine-Proline-Proline (IPP) are angiotensin converting enzyme inhibitors and may have potential to alleviate hypertension. The aim of this work was to understand the interactions of IPP and LKP when formulated into a chitosan nanoparticle (NP) to help improve permeation. Our findings indicate different mean inhibitory concentrations (LKP: 0.36 ± 0.01 μM and IPP: 3.1 ± 0.6 μM) and encapsulation efficiencies at different ratios of chitosan: tripolyphosphate (LKP NPs: 65% at 6:1 and IPP NPs: 43% at 4:1). Molecular modelling and circular dichroism showed different stable amino side-chain-specific conformations for each peptide. IPP showed more steric hindrances to intra-chain rotations, resulting in an unordered peptide structure, whereas LKP showed more flexibility associated with a (disordered) β-strand-like conformer. In-vitro release kinetics showed a slower release for LKP NPs in acidic pH compared to IPP NPs. In conclusion, LKP NPs were found to have better binding compatibility with chitosan.
Funding Details: Department of Agriculture, Food and the Marine
Type of material: Journal Article
Publisher: Elsevier
Journal: Innovative Food Science and Emerging Technologies
Volume: 44
Start page: 139
End page: 148
Copyright (published version): 2017 Elsevier
Keywords: Leucine-Lysine-Proline (LKP)Isoleucine-Proline-Proline (IPP)Bioactive peptidesChitosan nanoparticlesACE inhibitorsCircular dichroismIn-vitro release kinetics
DOI: 10.1016/j.ifset.2017.07.002
Language: en
Status of Item: Peer reviewed
Appears in Collections:Veterinary Medicine Research Collection

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