Exploring the Potential Application of Short Non‐Coding RNA‐Based Genetic Circuits in Chinese Hamster Ovary Cells

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Title: Exploring the Potential Application of Short Non‐Coding RNA‐Based Genetic Circuits in Chinese Hamster Ovary Cells
Authors: Valdés-Bango Curell, Ricardo
Barron, Niall
Permanent link: http://hdl.handle.net/10197/10564
Date: 29-Jan-2018
Online since: 2019-05-21T09:07:32Z
Abstract: The majority of cell engineering for recombinant protein production to date has relied on traditional genetic engineering strategies, such as gene overexpression and gene knock-outs, to substantially improve the production capabilities of Chinese Hamster Ovary (CHO) cells. However, further improvements in cellular productivity or control over product quality is likely to require more sophisticated rational approaches to coordinate and balance cellular pathways. For these strategies to be implemented, novel molecular tools need to be developed to facilitate more refined control of gene expression. Multiple gene control strategies are developed over the last decades in the field of synthetic biology, including DNA and RNA-based systems, which allows tight and timely control over gene expression. microRNAs has received a lot of attention over the last decade in the CHO field and are used to engineer and improve CHO cells. In this review we focus on microRNA-based gene control systems and discuss their potential use as tools rather than targets in order to gain better control over gene expression.
Funding Details: European Commission Horizon 2020
Type of material: Journal Article
Publisher: Wiley Online Library
Journal: Biotechnology Journal
Volume: 13
Issue: 10
Start page: e1700220
Copyright (published version): 2018 Wiley
Keywords: Cellular engineeringCHO cellsGenetic circuitsmiRNAProtein expression controlRNA interferenceSynthetic biology
DOI: 10.1002/biot.201700220
Language: en
Status of Item: Peer reviewed
Appears in Collections:Chemical and Bioprocess Engineering Research Collection

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