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Exploring the Potential Application of Short Non‐Coding RNA‐Based Genetic Circuits in Chinese Hamster Ovary Cells
Date Issued
2018-01-29
Date Available
2019-05-21T09:07:32Z
Abstract
The majority of cell engineering for recombinant protein production to date has relied on traditional genetic engineering strategies, such as gene overexpression and gene knock-outs, to substantially improve the production capabilities of Chinese Hamster Ovary (CHO) cells. However, further improvements in cellular productivity or control over product quality is likely to require more sophisticated rational approaches to coordinate and balance cellular pathways. For these strategies to be implemented, novel molecular tools need to be developed to facilitate more refined control of gene expression. Multiple gene control strategies are developed over the last decades in the field of synthetic biology, including DNA and RNA-based systems, which allows tight and timely control over gene expression. microRNAs has received a lot of attention over the last decade in the CHO field and are used to engineer and improve CHO cells. In this review we focus on microRNA-based gene control systems and discuss their potential use as tools rather than targets in order to gain better control over gene expression.
Sponsorship
European Commission Horizon 2020
Type of Material
Journal Article
Publisher
Wiley Online Library
Journal
Biotechnology Journal
Volume
13
Issue
10
Start Page
e1700220
Copyright (Published Version)
2018 Wiley
Language
English
Status of Item
Peer reviewed
ISSN
1860-6768
This item is made available under a Creative Commons License
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Name
BTJ-miRNA Review-RVB-final_corrected peer review_NB_Revised.pdf
Size
487.71 KB
Format
Owning collection
Scopus© citations
6
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