Delayed differentiation of vaginal and uterine microbiomes in dairy cows developing postpartum endometritis

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Title: Delayed differentiation of vaginal and uterine microbiomes in dairy cows developing postpartum endometritis
Authors: Miranda-CasoLuengo, Raúl
Lu, Junnan
Williams, Erin J.
Miranda-CasoLuengo, Aleksandra A.
Carrington, Stephen D.
Evans, Alexander C. O.
Meijer, Wim G.
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Date: 10-Jan-2019
Online since: 2019-05-21T11:07:09Z
Abstract: Bacterial overgrowth in the uterus is a normal event after parturition. In contrast to the healthy cow, animals unable to control the infection within 21 days after calving develop postpartum endometritis. Studies on the Microbial Ecology of the bovine reproductive tract have focused on either vaginal or uterine microbiomes. This is the first study that compares both microbiomes in the same animals. Terminal Restriction Fragment Length Polymorphism of the 16S rRNA gene showed that despite large differences associated to individuals, a shared community exist in vagina and uterus during the postpartum period. The largest changes associated with development of endometritis were observed at 7 days postpartum, a time when vaginal and uterine microbiomes were most similar. 16S rRNA pyrosequencing of the vaginal microbiome at 7 days postpartum showed at least three different microbiome types that were associated with later development of postpartum endometritis. All three microbiome types featured reduced bacterial diversity. Taken together, the above findings support a scenario where disruption of the compartmentalization of the reproductive tract during parturition results in the dispersal and mixing of the vaginal and uterine microbiomes, which subsequently are subject to differentiation. This differentiation was observed early postpartum in the healthy cow. In contrast, loss of bacterial diversity and dominance of the microbiome by few bacterial taxa were related to a delayed succession at 7DPP in cows that at 21 DPP or later were diagnosed with endometritis.
Funding Details: Department of Agriculture, Food and the Marine
Science Foundation Ireland
Type of material: Journal Article
Publisher: PLOS
Journal: PLoS ONE
Volume: 14
Issue: 1
Start page: e0200974
Copyright (published version): 2019 the Authors
Keywords: MicrobiomeVaginaUterusMucusBacteriaMicrobial taxonomyFusobacteriaMicrobial ecology
DOI: 10.1371/journal.pone.0200974
Language: en
Status of Item: Peer reviewed
Appears in Collections:Conway Institute Research Collection
Biomolecular and Biomedical Science Research Collection
Agriculture and Food Science Research Collection
Veterinary Medicine Research Collection

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