Production of Whey-Derived DPP-IV Inhibitory Peptides Using an Enzymatic Membrane Reactor

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Title: Production of Whey-Derived DPP-IV Inhibitory Peptides Using an Enzymatic Membrane Reactor
Authors: O’Halloran, John
O'Sullivan, Michael
Casey, Eoin
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Date: 4-Mar-2019
Online since: 2019-05-29T10:58:24Z
Abstract: Continuous processing in the production of peptides is an area of increased interest. In this study, an enzymatic membrane reactor (EMR) was developed whereby whey protein isolate was used as a substrate to prepare DPP-IV inhibitory and radical scavenging peptides via enzymatic hydrolysis. Two separate enzymes were tested: Corolase 2TS and Protamex in conventional batch processes and the EMR. Neither enzyme was considered effective at producing peptides with radical scavenging activity when measured using a DPPH assay. However, both enzymes were capable of producing DPP-IV inhibitory peptides. Corolase and Protamex both produced similar DPP-IV inhibition levels upon completion of batch experiments. In the EMR process, permeate in the Protamex run showed 33.7% lower IC50 value compared to the continuous Corolase run. Protamex was a better enzyme at producing the DPP-IV inhibitory effect. The continuous (EMR) production method showed an increased productivity over batch for both enzymes.
Funding Details: Enterprise Ireland
Type of material: Journal Article
Publisher: Springer
Journal: Food and Bioprocess Technology
Volume: 12
Issue: 5
Start page: 799
End page: 808
Copyright (published version): 2019 Springer Nature
Keywords: Enzymatic membrane reactorDPP-IV inhibitory peptidesPeptides with specific bioactivityWhey protein
DOI: 10.1007/s11947-019-02253-7
Language: en
Status of Item: Peer reviewed
Appears in Collections:Chemical and Bioprocess Engineering Research Collection
Agriculture and Food Science Research Collection

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