Development of a bacterial propionate-biosensor for anaerobic digestion monitoring

DC FieldValueLanguage
dc.contributor.authorSweeney, Joseph B.-
dc.contributor.authorMurphy, Cormac D.-
dc.contributor.authorMcDonnell, Kevin-
dc.date.accessioned2019-07-01T13:46:43Z-
dc.date.available2019-07-01T13:46:43Z-
dc.date.copyright2017 Elsevieren_US
dc.date.issued2018-02-01-
dc.identifier.citationEnzyme and Microbial Technologyen_US
dc.identifier.issn0141-0229-
dc.identifier.urihttp://hdl.handle.net/10197/10829-
dc.description.abstractMonitoring anaerobic digestion (AD) leachate for changes in acetate and propionate concentrations is essential for effective AD operation. In this paper the development of a novel propionate cell-based biosensor is described. A previously designed E. coli mutant (IMD Wldgy) that could selectively determine acetate concentrations in synthetic leachates, based on oxygen uptake measurements, was used as a starting point in the development of a propionate biosensor. However, the propionate-grown IMD Wldgy cells exhibited extremely low propionate:acetate O2 consumption ratios (1:2.4). Screening for alternative propionate-grown E. coli strains naturally possessing a more favourable propionate:acetate O2 consumption ratio identified strain IMD 1, which exhibited a positive ratio (1.6:1). To improve the selectivity of the strain, successive gene knockouts were performed generating the IMD 1 hldgyep mutant. However, propionate-grown IMD 1hdlgyep's O2 consumption ratio was deemed too low to be considered as a propionate detecting bio-element. It was reasoned that the mechanisms by which E. coli activates acetate had to be removed. Deleting acs (acetyl-CoA synthesase) and ackA (acetate kinase) from IMD Wldgyep, resulted in an E. coli IMD Wldgyepak knockout mutant that, when grown on propionate, produced a mean propionate:acetate O2 consumption ratio of approx. 13:1. The resulting IMD Wldgyep and IMD Wldgyepak strains, which formed the acetate- and propionate-biosensor, respectively, were capable of detecting acetate and propionate concentrations ranging from 0.05 mM to 4.5 mM within two-phase AD synthetic leachates.en_US
dc.description.sponsorshipScience Foundation Irelanden_US
dc.format.mediumPrint-Electronic-
dc.language.isoenen_US
dc.publisherElsevier BVen_US
dc.rightsThis is the author’s version of a work that was accepted for publication in Enzyme and Microbial Technology. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Enzyme and Microbial Technology (109, (2018)) https://doi.org/10.1016/j.enzmictec.2017.09.011en_US
dc.subjectEscherichia colien_US
dc.subjectOxygenen_US
dc.subjectAcetatesen_US
dc.subjectAcsen_US
dc.subjectBiosensoren_US
dc.subjectAnaerobic digestionen_US
dc.subjectDigestionen_US
dc.subjectMutationen_US
dc.subjectAckAen_US
dc.subjectVFAen_US
dc.subjectMonitoring and controlen_US
dc.subjectSynthetic leachateen_US
dc.titleDevelopment of a bacterial propionate-biosensor for anaerobic digestion monitoringen_US
dc.typeJournal Articleen_US
dc.internal.authorcontactothercormac.d.murphy@ucd.ieen_US
dc.statusPeer revieweden_US
dc.identifier.volume109en_US
dc.identifier.startpage51en_US
dc.identifier.endpage57en_US
dc.identifier.doi10.1016/j.enzmictec.2017.09.011-
dc.neeo.contributorSweeney|Joseph B.|aut|-
dc.neeo.contributorMurphy|Cormac D.|aut|-
dc.neeo.contributorMcDonnell|Kevin|aut|-
dc.description.othersponsorshipCharles Parsons Energy Research Programmeen_US
dc.date.updated2019-06-25T11:31:34Z-
dc.identifier.grantid06/CP/E001-
item.fulltextWith Fulltext-
item.grantfulltextopen-
Appears in Collections:Biomolecular and Biomedical Science Research Collection
Files in This Item:
File Description SizeFormat 
REVISION Propionate biosensor EMT CLEAN.docx310.66 kBUnknownDownload
Show simple item record

SCOPUSTM   
Citations 50

2
Last Week
0
Last month
checked on Oct 20, 2019

Google ScholarTM

Check

Altmetric


This item is available under the Attribution-NonCommercial-NoDerivs 3.0 Ireland. No item may be reproduced for commercial purposes. For other possible restrictions on use please refer to the publisher's URL where this is made available, or to notes contained in the item itself. Other terms may apply.