Virus-induced gene silencing (VIGS) for functional characterization of disease resistance genes in barley seedlings

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Title: Virus-induced gene silencing (VIGS) for functional characterization of disease resistance genes in barley seedlings
Authors: Gunupuru, Lokanadha R.Perochon, AlexandreAli, Shahin S.Scofield, Steven R.Doohan, Fiona M.
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Date: 2019
Online since: 2019-09-09T11:23:58Z
Abstract: With the recent advances in sequencing technologies, many studies are generating lists of candidate genes associated with specific traits. The major bottleneck in functional genomics is the validation of gene function. This is achieved by analyzing the effect of either gene silencing or overexpression on a specific phenotypic or biochemical trait. This usually requires the generation of stable transgenic plants and this can take considerable time. Therefore any technique that expedites the validation of gene function is of particular benefit in cereals, including barley. One such technique is Virus-Induced Gene Silencing (VIGS), which evokes a natural antiviral defense mechanism in plants. VIGS can be used to downregulate gene expression in a transient manner, but long enough to determine its effects on a specific phenotype. It is particularly useful for screening candidate genes and selecting those with potential for disease control. VIGS based on Barley Stripe Mosaic Virus (BSMV) is a powerful and efficient tool for the analysis of gene function in cereals. Here we present a BSMV VIGS protocol for simple and robust gene silencing in barley and describe it to evaluate the role of the hormone receptor BRI1 (Brassinosteroid Insensitive 1) in barley leaf resistance to Fusarium infection.
Funding Details: Department of Agriculture, Food and the Marine
Science Foundation Ireland
Type of material: Book Chapter
Publisher: Springer New York
Volume: 1900
Start page: 95
End page: 114
Copyright (published version): 2019 Springer
Keywords: Plant virusesFungal sporesFusariumHordeumSeedsPlant leavesOxidoreductasesViral RNABiological assayReproducibility of resultsGerminationGenetic transcriptionGene silencingPlant genesPlant genomeGenetic vectorsPlasmidsDisease resistancePlant developmentSeedlings
DOI: 10.1007/978-1-4939-8944-7_7
Language: en
Status of Item: Peer reviewed
Is part of: Harwood W. (ed.). Barley. Methods in Molecular Biology, vol 1900. Humana Press, New York, NY
Appears in Collections:Biology & Environmental Science Research Collection
Earth Institute Research Collection

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