Non-destructive determination of collagen fibril width in extruded collagen fibres by piezoresponse force microscopy

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Title: Non-destructive determination of collagen fibril width in extruded collagen fibres by piezoresponse force microscopy
Authors: Bazaid, ArwaNeumayer, Sabine M.Sorushanova, AnnaGuyonnet, JillZeugolis, Dimitrios I.Rodriguez, Brian J.
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Date: 13-Sep-2017
Online since: 2019-11-13T09:01:45Z
Abstract: Extruded collagen fibres are a promising platform for tissue engineering applications. Ensuring that the functional properties of the engineered tissues possess similar structural properties as native tissues is important for biomedical applications. Advanced imaging tools including scanning electron microscopy (SEM) and atomic force microscopy (AFM) have revealed the structural features of collagen fibrils within such fibres; however, these techniques often require modification steps that can alter the sample in the process. Here, lateral piezoresponse force microscopy (LPFM), which is sensitive to the polar orientation of piezoelectric collagen fibrils, is demonstrated as a promising tool to assess the width of individual fibrils and moreover map their organisation and polar orientation without altering the sample. Within the fibres studied, the collagen fibrils showed a highly anisotropic arrangement with preferred alignment along the length of the fibre. Fibril widths of 74 ± 18 nm and 73 ± 19 nm in untreated and bleached fibres, respectively, were measured from LPFM amplitude images. These values agreed with values from SEM (70 ± 10 nm) and AFM (71 ± 19 nm) measurements that could only be obtained from bleached fibres.
Funding Details: Department of Agriculture, Food and the Marine
European Commission Horizon 2020
Science Foundation Ireland
metadata.dc.description.othersponsorship: Ministry of Higher Education of Saudi Arabia
Type of material: Journal Article
Publisher: IOP Publishing
Journal: Biomedical Physics and Engineering Express
Volume: 3
Issue: 5
Copyright (published version): 2017 IOP Publishing
Keywords: CollagenBiomaterialsExtrustionAFMPFMPiezoelectricityElectromechanical coupling
DOI: 10.1088/2057-1976/aa85ec
Language: en
Status of Item: Peer reviewed
Appears in Collections:Conway Institute Research Collection
Physics Research Collection

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