Evaluation of Demodex mite viability using motility and scattered light intensity
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|Title:||Evaluation of Demodex mite viability using motility and scattered light intensity||Authors:||Gatault, Solène; Foley, Ruth; Shiels, L.; Powell, Frank C.||Permanent link:||http://hdl.handle.net/10197/11200||Date:||19-Apr-2019||Online since:||2019-11-14T13:00:47Z||Abstract:||Demodex mites have been suggested to have a role in various cutaneous and ocular disorders pathogenesis, such as rosacea or blepharitis. Evaluation of potential treatments with anti-Demodex effects is difficult because the viability of living mites needs to be evaluated during their exposure to the agent being tested. Mite viability is currently based solely on their observed movement. However, this method of assessing viability has significant limitations as mites may be resting, immobile or paralysed at any given observation point giving the observer a false impression of the organism’s death. To overcome this limitation we evaluated a new quantitative method of evaluating the viability of Demodex mites by using scattered light intensity (SLI). We demonstrated that when combined with observation of mite motility, SLI provided increased accuracy of the evaluation of viability of mites being studied. This new viability assay will help address the technical challenges of mite viability experiments. Accurate evaluation of mite viability will enhance mite biology research and allow for more accurate in vitro toxicity assays of proposed anti-mite agents.||Funding Details:||European Commission Horizon 2020||Type of material:||Journal Article||Publisher:||Springer||Journal:||Experimental and Applied Acarology||Volume:||77||Issue:||4||Start page:||463||End page:||469||Copyright (published version):||2019 Springer||Keywords:||Entomology; Scaterred light intensity; Demodex; Viability assay; Rosacea; Demodicosis; Blepharitis||DOI:||10.1007/s10493-019-00358-4||Language:||en||Status of Item:||Peer reviewed|
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