Migration of Fasciola hepatica newly excysted juveniles is inhibited by high-mannose and oligomannose-Type N-glycan-binding lectins

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Title: Migration of Fasciola hepatica newly excysted juveniles is inhibited by high-mannose and oligomannose-Type N-glycan-binding lectins
Authors: Garcia-Campos, AndresBaird, Alan W.Mulcahy, Grace
Permanent link: http://hdl.handle.net/10197/11233
Date: 1-Nov-2017
Online since: 2019-12-06T14:59:39Z
Abstract: Fasciola hepatica has both zoonotic importance and high economic impact in livestock worldwide. After ingestion by the definitive host, the Newly Excysted Juveniles (NEJ) penetrate the intestine before reaching the peritoneal cavity. The role of some NEJ-derived proteins in invasion has been documented, but the role of NEJ glycans or lectin-binding receptors during initial infection in the gut is still unknown. To address these questions, the migration of NEJ through rat intestine was recorded at 30 min intervals up to 150 min by two ex vivo methods. Firstly, jejunal sheets were challenged with NEJ incubated with biotinylated lectins. Secondly, untreated NEJ were incubated with distal jejunum pre-Treated with lectins. Both Concanavalin A (ConA) and Galanthus nivalis (GNL), which recognize mannose-Type N-glycans, significantly inhibited NEJ migration across the jejunum. Most of the lectins bound to the tegument and oral sucker of the NEJ, but only ConA and GNL maintained this interaction over 150 min. None of the lectins examined significantly reduced NEJ migration when pre-incubated with jejunal sheets, suggesting that host glycans might not be essential for initial binding/recognition of the gut by NEJ. Agents capable of blocking mannose-Type N-glycans on the NEJ tegument may have potential for disrupting infection.
Funding Details: European Commission Horizon 2020
Type of material: Journal Article
Publisher: Cambridge University Press
Journal: Parasitology
Volume: 144
Issue: 13
Start page: 1708
End page: 1717
Copyright (published version): 2015 Cambridge University Press
Keywords: AnimalsRats, WistarFasciola hepaticaFascioliasisPolysaccharidesLectinsMaleHost-parasite interactions
DOI: 10.1017/S003118201700124X
Language: en
Status of Item: Peer reviewed
Appears in Collections:Conway Institute Research Collection
Veterinary Medicine Research Collection

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