Histochemistry evaluation of the oxidative stress and the antioxidant status in Cu-supplemented cattle

Files in This Item:
File Description SizeFormat 
Histochemistry evaluation of the oxidative stress and the antioxidant status in Cu.docx455.98 kBUnknownDownload
Title: Histochemistry evaluation of the oxidative stress and the antioxidant status in Cu-supplemented cattle
Authors: García-Vaquero, MarcoBenedito, José LuisLópez-Alonso, MartaMiranda, Marta
Permanent link: http://hdl.handle.net/10197/11299
Date: Sep-2012
Online since: 2020-03-05T15:48:08Z
Abstract: The aim of this paper is to evaluate at a histopathological level the effect of the most commonly used copper (Cu) supplementation (15 mg/kg dry matter (DM)) in the liver of intensively reared beef cattle. This was done by a histochemistry evaluation of (i) the antioxidant capacity in the liver - by the determination of metallothioneins (MT) and superoxide dismutase (SOD) expression - as well as (ii) the possible induction of oxidative damage - by the determination of inducible nitric oxide synthase (iNOS), nitrotyrosine (NITT), malondialdehyde (MDA) and 8-oxoguanine (8-oxo) - that (iii) could increase apoptotic cell death - determined by cytochrome-c (cyto-c), caspase 1 (casp1) and terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL). Liver samples from Cu-supplemented (15 mg Cu sulphate/kg DM, n = 5) and non-supplemented calves (n = 5) that form part of other experiments to evaluate Cu status were collected at slaughter and processed for immunohistochemistry and TUNEL. MT expression was diffuse and SOD showed slight changes although without statistical significance. iNOS and NITT positive (+) cells significantly increased, mainly around the central veins in the animals from the Cu-supplemented group, whereas no differences were appreciated for the rest of the oxidative stress and apoptosis markers. Under the conditions of this study, which are the conditions of the cattle raised in intensive systems in NW Spain and also many European countries, routinely Cu supplementation increased the risk of the animals to undergo subclinical Cu toxicity, with no significant changes in the Cu storage capacity and the antioxidant defensive system evaluated by MT and SOD expression, but with a significant and important increase of oxidative damage measured by iNOS and NITT. The results of this study indicated that iNOS and NITT could be used as early markers of initial pathological changes in the liver caused by Cu supplementation in cattle, although more studies in cattle under different levels of Cu supplementation are needed.
metadata.dc.description.othersponsorship: Xunta de Galicia (Spain)
Type of material: Journal Article
Publisher: Cambridge University Press
Journal: Animal
Volume: 6
Issue: 9
Start page: 1435
End page: 1443
Copyright (published version): 2012 The Animal Consortium
Keywords: LiverAnimalsCattleCopperAntioxidantsApoptosisOxidative stressAnimal husbandryAnimal feedDietary supplementsSpain
DOI: 10.1017/S1751731112000535
Language: en
Status of Item: Peer reviewed
Appears in Collections:Agriculture and Food Science Research Collection

Show full item record

Citations 20

Last Week
Last month
checked on Mar 21, 2020

Page view(s)

Last Week
Last month
checked on Mar 28, 2020


checked on Mar 28, 2020

Google ScholarTM



This item is available under the Attribution-NonCommercial-NoDerivs 3.0 Ireland. No item may be reproduced for commercial purposes. For other possible restrictions on use please refer to the publisher's URL where this is made available, or to notes contained in the item itself. Other terms may apply.