The involvement of metallothionein in hepatic and renal Cd, Cu and Zn accumulation in pigs
Files in This Item:
|The involvement of metallothionein in hepatic and renal Cd.docx||197.15 kB||Unknown||Download|
|Title:||The involvement of metallothionein in hepatic and renal Cd, Cu and Zn accumulation in pigs||Authors:||López-Alonso, Marta; Benedito, José Luis; García-Vaquero, Marco; Hernández, Joaquin; Miranda, Marta||Permanent link:||http://hdl.handle.net/10197/11300||Date:||Dec-2012||Online since:||2020-03-05T16:03:03Z||Abstract:||This study investigated the involvement of metallothionein (MT) in hepatic and renal cadmium (Cd) accumulation and the interactions of this element with the essential elements copper (Cu) and zinc (Zn) in pigs receiving diets with or without Cu and Zn supplementation, in intensive and extensive production systems respectively. Animals from intensive systems showed significantly higher Cd concentrations in the liver (83.3 μg/kg wet weight) and kidney (343 μg/kg) than animals from extensive systems (33.2 and 130 μg/kg respectively). Cu (liver 16.9, kidney 5.52. mg/kg) and Zn (82.8 and 29.7. mg/kg) concentrations were also significantly higher in pigs from intensive than in animals from extensive systems (Cu 10.1 and 4.64. mg/kg, Zn 66.2 and 23.1. mg/kg). Pigs from intensive systems showed 50% higher kidney MT concentrations than animals from extensive systems (278 and 183. mg/kg respectively), whereas liver MT concentrations were very similar in the two groups (1696 and 1517. mg/kg respectively). MT concentrations in both the liver and the kidney were strongly dependent on the Zn status of the animal. In the liver neither Cu nor Cd displaced Zn from MT, and the proportion of MT binding sites apparently occupied by Cu and Cd decreased with increasing hepatic MT concentration, despite the fact that both Cu and Cd have higher affinity for MT than Zn. The proportion of MT binding sites occupied by Cu and Cd was also directly related to Zn:Cu ratio in hepatic cells. In the kidney, in contrast, Cu seems able to compete with Zn for MT binding sites, and the proportion of MT binding sites occupied by Cu increased with increasing renal MT concentration. © 2012 Elsevier B.V.||metadata.dc.description.othersponsorship:||Xunta de Galicia (Spain)||Type of material:||Journal Article||Publisher:||Elsevier||Journal:||Livestock Science||Volume:||150||Issue:||1-3||Start page:||152||End page:||158||Copyright (published version):||2012 Elsevier||Keywords:||Agriculture; Toxic metals; Trace elements; Metallothionein; Pigs; Extensive; Intensive; Cadmium retention; Messenger RNA; Zinc status; Copper; Metabolism; Protein; Supplementation; Exposure; Kidneys; Tissues||DOI:||10.1016/j.livsci.2012.08.012||Language:||en||Status of Item:||Peer reviewed|
|Appears in Collections:||Agriculture and Food Science Research Collection|
Show full item record
This item is available under the Attribution-NonCommercial-NoDerivs 3.0 Ireland. No item may be reproduced for commercial purposes. For other possible restrictions on use please refer to the publisher's URL where this is made available, or to notes contained in the item itself. Other terms may apply.