In vitro and in silico approaches to generating and identifying angiotensin-converting enzyme I inhibitory peptides from green macroalga ulva lactuca

DC FieldValueLanguage
dc.contributor.authorGarcía-Vaquero, Marco-
dc.contributor.authorMora, Leticia-
dc.contributor.authorHayes, Maria-
dc.date.accessioned2020-05-22T11:43:58Z-
dc.date.available2020-05-22T11:43:58Z-
dc.date.copyright2019 the Authorsen_US
dc.date.issued2019-03-30-
dc.identifier.citationMarine Drugsen_US
dc.identifier.issn1660-3397-
dc.identifier.urihttp://hdl.handle.net/10197/11378-
dc.description.abstractA protein extract was generated from the macroalga Ulva lactuca, which was subsequently hydrolysed using the food-grade enzyme papain and angiotensin-converting Enzyme I and renin inhibitory peptides identified using a combination of enrichment strategies employing molecular weight cutoff filtration and mass spectrometry analysis. The generated hydrolysates with the most promising in vitro activity were further purified using preparative RP-HPLC and characterised. The 1 kDa hydrolysate (1 kDa-UFH), purified and collected by preparative RP-HPLC at minutes 41-44 (Fr41-44), displayed statistically higher ACE-I inhibitory activities ranging from 96.91% to 98.06%. A total of 48 novel peptides were identified from these four fractions by LC-MS/MS. A simulated gastrointestinal digestion of the identified peptide sequences was carried out using in silico enzyme cleavage simulation tools, resulting in 86 peptide sequences that were further assessed for their potential activity, toxicity and allergenicity using multiple predictive approaches. All the peptides obtained in this study were predicted to be non-toxic. However, 28 out of the 86 novel peptides released after the in silico gastrointestinal digestion were identified as potential allergens. The potential allergenicity of these peptides should be further explored to comply with the current labelling regulations in formulated food products containing U. lactuca protein hydrolysates.en_US
dc.description.sponsorshipScience Foundation Irelanden_US
dc.description.sponsorshipTeagascen_US
dc.format.mediumElectronic-
dc.language.isoenen_US
dc.publisherMDPIen_US
dc.rightsThis is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.en_US
dc.subjectHumansen_US
dc.subjectUlvaen_US
dc.subjectSeaweeden_US
dc.subjectOligopeptidesen_US
dc.subjectProtein hydrolysatesen_US
dc.subjectAntihypertensive agentsen_US
dc.subjectAllergensen_US
dc.subjectAngiotensin-Converting Enzyme Inhibitorsen_US
dc.subjectAmino acid sequenceen_US
dc.subjectHydrolysisen_US
dc.subjectComputer simulationen_US
dc.titleIn vitro and in silico approaches to generating and identifying angiotensin-converting enzyme I inhibitory peptides from green macroalga ulva lactucaen_US
dc.typeJournal Articleen_US
dc.internal.authorcontactothermarco.garciavaquero@ucd.ieen_US
dc.statusPeer revieweden_US
dc.identifier.volume17en_US
dc.identifier.issue4en_US
dc.citation.otherArticle Number: 204en_US
dc.identifier.doi10.3390/md17040204-
dc.neeo.contributorGarcía-Vaquero|Marco|aut|-
dc.neeo.contributorMora|Leticia|aut|-
dc.neeo.contributorHayes|Maria|aut|-
dc.description.othersponsorshipThe Barrié Foundation in Galiciaen_US
dc.date.updated2020-04-17T16:19:14Z-
dc.identifier.grantid14/IA/2548-
dc.identifier.grantidNFNY6889-142-
item.fulltextWith Fulltext-
item.grantfulltextopen-
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