Wing membrane biopsies for bat cytogenetics: Finding of 2n = 54 in irish Rhinolophus hipposideros (rhinolophidae, chiroptera, mammalia) supports two geographically separated chromosomal variants in Europe

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Title: Wing membrane biopsies for bat cytogenetics: Finding of 2n = 54 in irish Rhinolophus hipposideros (rhinolophidae, chiroptera, mammalia) supports two geographically separated chromosomal variants in Europe
Authors: Kacprzyk, JoannaTeeling, Emma C.Kelleher, C. (Conor)Volleth, Marianne
Permanent link: http://hdl.handle.net/10197/11576
Date: 1-Aug-2016
Online since: 2020-09-22T10:00:56Z
Abstract: In Europe, 2 different diploid chromosome numbers, 2n = 54 and 2n = 56, have been described in the lesser horseshoe bat (Rhinolophushipposideros). The eastern form with 2n = 56 extends from the Czech Republic to Greece. To date, specimens with 54 chromosomes have been reported only from Spain and Germany. This study expands the distributional area of the western variant to Ireland. Strikingly, this distribution of European chromosomal variants is in contrast to the available molecular data that indicate little genetic differentiation of R. hipposideros populations spanning Northwestern to Central Europe. Further, we have developed an optimized protocol for establishing fibroblast cell cultures, suitable for karyotype analyses, from 3-mm wing membrane biopsies. This is a useful technique for cytogenetic studies of endangered bat species, as this non-lethal sampling method imposes only minimum stress to the animal without lasting adverse effects and is routinely used to sample tissue probes for molecular genetic studies in bats.
Funding Details: European Research Council
University College Dublin
Type of material: Journal Article
Publisher: Karger
Journal: Cytogenetic and Genome Research
Volume: 148
Issue: 4
Start page: 279
End page: 283
Copyright (published version): 2016 Karger Publishers
Keywords: FibroblastsAnimalsChiropteraBiopsyCell Culture TechniquesKaryotypingEcosystemDiploidyIrelandFemaleMaleWings, Animal
DOI: 10.1159/000447111
Language: en
Status of Item: Peer reviewed
Appears in Collections:Biology & Environmental Science Research Collection

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