Wing membrane biopsies for bat cytogenetics: Finding of 2n = 54 in irish Rhinolophus hipposideros (rhinolophidae, chiroptera, mammalia) supports two geographically separated chromosomal variants in Europe

DC FieldValueLanguage
dc.contributor.authorKacprzyk, Joanna-
dc.contributor.authorTeeling, Emma C.-
dc.contributor.authorKelleher, C. (Conor)-
dc.contributor.authorVolleth, Marianne-
dc.date.accessioned2020-09-22T10:00:56Z-
dc.date.available2020-09-22T10:00:56Z-
dc.date.copyright2016 Karger Publishersen_US
dc.date.issued2016-08-01-
dc.identifier.citationCytogenetic and Genome Researchen_US
dc.identifier.issn1424-8581-
dc.identifier.urihttp://hdl.handle.net/10197/11576-
dc.description.abstractIn Europe, 2 different diploid chromosome numbers, 2n = 54 and 2n = 56, have been described in the lesser horseshoe bat (Rhinolophushipposideros). The eastern form with 2n = 56 extends from the Czech Republic to Greece. To date, specimens with 54 chromosomes have been reported only from Spain and Germany. This study expands the distributional area of the western variant to Ireland. Strikingly, this distribution of European chromosomal variants is in contrast to the available molecular data that indicate little genetic differentiation of R. hipposideros populations spanning Northwestern to Central Europe. Further, we have developed an optimized protocol for establishing fibroblast cell cultures, suitable for karyotype analyses, from 3-mm wing membrane biopsies. This is a useful technique for cytogenetic studies of endangered bat species, as this non-lethal sampling method imposes only minimum stress to the animal without lasting adverse effects and is routinely used to sample tissue probes for molecular genetic studies in bats.en_US
dc.description.sponsorshipEuropean Research Councilen_US
dc.description.sponsorshipUniversity College Dublinen_US
dc.format.mediumPrint-Electronic-
dc.language.isoenen_US
dc.publisherKargeren_US
dc.rightsThe final, published version of this article is available at https://www.karger.com/?doi=10.1159/000447111en_US
dc.subjectFibroblastsen_US
dc.subjectAnimalsen_US
dc.subjectChiropteraen_US
dc.subjectBiopsyen_US
dc.subjectCell Culture Techniquesen_US
dc.subjectKaryotypingen_US
dc.subjectEcosystemen_US
dc.subjectDiploidyen_US
dc.subjectIrelanden_US
dc.subjectFemaleen_US
dc.subjectMaleen_US
dc.subjectWings, Animalen_US
dc.titleWing membrane biopsies for bat cytogenetics: Finding of 2n = 54 in irish Rhinolophus hipposideros (rhinolophidae, chiroptera, mammalia) supports two geographically separated chromosomal variants in Europeen_US
dc.typeJournal Articleen_US
dc.internal.authorcontactotherjoanna.kacprzyk@ucd.ieen_US
dc.statusPeer revieweden_US
dc.identifier.volume148en_US
dc.identifier.issue4en_US
dc.identifier.startpage279en_US
dc.identifier.endpage283en_US
dc.identifier.doi10.1159/000447111-
dc.neeo.contributorKacprzyk|Joanna|aut|-
dc.neeo.contributorTeeling|Emma C.|aut|-
dc.neeo.contributorKelleher|C. (Conor)|aut|-
dc.neeo.contributorVolleth|Marianne|aut|-
dc.date.updated2020-09-21T13:59:45Z-
item.fulltextWith Fulltext-
item.grantfulltextopen-
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