The retraction of the protoplast during PCD is an active, and interruptible, calcium-flux driven process

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dc.contributor.authorKacprzyk, Joanna-
dc.contributor.authorBrogan, Niall P.-
dc.contributor.authorDaly, Cara T.-
dc.contributor.authorDoyle, Siamsa M.-
dc.contributor.authorDiamond, Mark-
dc.contributor.authorMolony, Elizabeth M.-
dc.contributor.authorMcCabe, Paul F.- Elsevieren_US
dc.identifier.citationPlant Scienceen_US
dc.description.abstractThe protoplast retracts during apoptosis-like programmed cell death (AL-PCD) and, if this retraction is an active component of AL-PCD, it should be used as a defining feature for this type of programmed cell death. We used an array of pharmacological and genetic tools to test if the rates of protoplast retraction in cells undergoing AL-PCD can be modulated. Disturbing calcium flux signalling, ATP synthesis and mitochondrial permeability transition all inhibited protoplast retraction and often also the execution of the death programme. Protoplast retraction can precede loss of plasma membrane integrity and cell death can be interrupted after the protoplast retraction had already occurred. Blocking calcium influx inhibited the protoplast retraction, reduced DNA fragmentation and delayed death induced by AL-PCD associated stresses. At higher levels of stress, where cell death occurs without protoplast retraction, blocking calcium flux had no effect on the death process. The results therefore strongly suggest that retraction of the protoplast is an active biological process dependent on an early Ca2+-mediated trigger rather than cellular disintegration due to plasma membrane damage. Therefore this morphologically distinct cell type is a quantifiable feature, and consequently, reporter of AL-PCD.en_US
dc.description.sponsorshipIrish Research Councilen_US
dc.rightsThis is the author’s version of a work that was accepted for publication in Plant Science. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Plant Science (260, (2017)) DOI:10.1016/j.plantsci.2017.04.001en_US
dc.subjectPlant proteinsen_US
dc.subjectSignal transductionen_US
dc.subjectCell deathen_US
dc.subjectDNA fragmentationen_US
dc.titleThe retraction of the protoplast during PCD is an active, and interruptible, calcium-flux driven processen_US
dc.typeJournal Articleen_US
dc.statusPeer revieweden_US
dc.neeo.contributorBrogan|Niall P.|aut|-
dc.neeo.contributorDaly|Cara T.|aut|-
dc.neeo.contributorDoyle|Siamsa M.|aut|-
dc.neeo.contributorMolony|Elizabeth M.|aut|-
dc.neeo.contributorMcCabe|Paul F.|aut|-
dc.description.othersponsorshipUCD School of Biology and Environmental Science postgraduate funding awarden_US
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