A root hair assay to expedite cell death research
|Title:||A root hair assay to expedite cell death research||Other Titles:||A Root Hair Assay||Authors:||Kacprzyk, Joanna; McCabe, Paul F.||Permanent link:||http://hdl.handle.net/10197/11579||Date:||1-Jan-2015||Online since:||2020-09-22T10:59:40Z||Abstract:||Programmed cell death can be defined as an organized cellular destruction and can be activated throughout plant development, as a defense response against invading pathogens or during environmental stress. The root hair assay presented herein enables in vivo quantitative measurements of programmed cell death based on the morphological changes of dying root hairs. Application of this novel, simple technique eliminates the need for establishing cell suspension cultures, resulting in a significant reduction in time, cost, and labor input. Here, we present a detailed root hair assay protocol for the dicotyledonous model plant Arabidopsis thaliana, where results from germination to scoring of cell death can be obtained within 7 days. We also suggest and present a panel of cell death inducing treatments which can be used to study abiotic stress- and mycotoxin-induced programmed cell death in the root hair system in Arabidopsis. A root hair assay protocol for the monocotyledonous model species Brachypodium distachyon is also included.||Funding Details:||Irish Research Council for Science, Engineering and Technology||Type of material:||Book Chapter||Publisher:||Humana Press and Springer||Start page:||73||End page:||82||Copyright (published version):||2015 Springer||Keywords:||Arabidopsis; Plant roots; Cytological techniques; Cell death; Germination; Brachypodium||DOI:||10.1007/978-1-4939-1902-4_7||Language:||en||Status of Item:||Peer reviewed||Is part of:||Estevez JM. (ed.). Plant Cell Expansion. Methods in Molecular Biology (Methods and Protocols), vol 1242||ISBN:||9781493919017|
|Appears in Collections:||Biology & Environmental Science Research Collection|
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