A root hair assay to expedite cell death research

DC FieldValueLanguage
dc.contributor.authorKacprzyk, Joanna-
dc.contributor.authorMcCabe, Paul F.-
dc.date.accessioned2020-09-22T10:59:40Z-
dc.date.available2020-09-22T10:59:40Z-
dc.date.copyright2015 Springeren_US
dc.date.issued2015-01-01-
dc.identifier.isbn9781493919017-
dc.identifier.urihttp://hdl.handle.net/10197/11579-
dc.description.abstractProgrammed cell death can be defined as an organized cellular destruction and can be activated throughout plant development, as a defense response against invading pathogens or during environmental stress. The root hair assay presented herein enables in vivo quantitative measurements of programmed cell death based on the morphological changes of dying root hairs. Application of this novel, simple technique eliminates the need for establishing cell suspension cultures, resulting in a significant reduction in time, cost, and labor input. Here, we present a detailed root hair assay protocol for the dicotyledonous model plant Arabidopsis thaliana, where results from germination to scoring of cell death can be obtained within 7 days. We also suggest and present a panel of cell death inducing treatments which can be used to study abiotic stress- and mycotoxin-induced programmed cell death in the root hair system in Arabidopsis. A root hair assay protocol for the monocotyledonous model species Brachypodium distachyon is also included.en_US
dc.description.sponsorshipIrish Research Council for Science, Engineering and Technologyen_US
dc.format.mediumPrint-
dc.language.isoenen_US
dc.publisherHumana Press and Springeren_US
dc.relation.ispartofEstevez JM. (ed.). Plant Cell Expansion. Methods in Molecular Biology (Methods and Protocols), vol 1242en_US
dc.rightsThe final publication is available at Springer via https://doi.org/10.1007/978-1-4939-1902-4_7en_US
dc.subjectArabidopsisen_US
dc.subjectPlant rootsen_US
dc.subjectCytological techniquesen_US
dc.subjectCell deathen_US
dc.subjectGerminationen_US
dc.subjectBrachypodiumen_US
dc.titleA root hair assay to expedite cell death researchen_US
dc.title.alternativeA Root Hair Assayen_US
dc.typeBook Chapteren_US
dc.internal.authorcontactotherjoanna.kacprzyk@ucd.ieen_US
dc.statusPeer revieweden_US
dc.identifier.startpage73en_US
dc.identifier.endpage82en_US
dc.identifier.doi10.1007/978-1-4939-1902-4_7-
dc.neeo.contributorKacprzyk|Joanna|aut|-
dc.neeo.contributorMcCabe|Paul F.|aut|-
dc.date.updated2020-09-22T10:35:11Z-
item.grantfulltextopen-
item.fulltextWith Fulltext-
Appears in Collections:Biology & Environmental Science Research Collection
Files in This Item:
File Description SizeFormat 
Kacprzyk and McCabe.pdf203.55 kBAdobe PDFDownload
Show simple item record

Page view(s)

132
Last Week
6
Last month
checked on Nov 28, 2020

Download(s)

25
checked on Nov 28, 2020

Google ScholarTM

Check

Altmetric


This item is available under the Attribution-NonCommercial-NoDerivs 3.0 Ireland. No item may be reproduced for commercial purposes. For other possible restrictions on use please refer to the publisher's URL where this is made available, or to notes contained in the item itself. Other terms may apply.