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A root hair assay to expedite cell death research
Alternative Title
A Root Hair Assay
Author(s)
Date Issued
2015-01-01
Date Available
2020-09-22T10:59:40Z
Abstract
Programmed cell death can be defined as an organized cellular destruction and can be activated throughout plant development, as a defense response against invading pathogens or during environmental stress. The root hair assay presented herein enables in vivo quantitative measurements of programmed cell death based on the morphological changes of dying root hairs. Application of this novel, simple technique eliminates the need for establishing cell suspension cultures, resulting in a significant reduction in time, cost, and labor input. Here, we present a detailed root hair assay protocol for the dicotyledonous model plant Arabidopsis thaliana, where results from germination to scoring of cell death can be obtained within 7 days. We also suggest and present a panel of cell death inducing treatments which can be used to study abiotic stress- and mycotoxin-induced programmed cell death in the root hair system in Arabidopsis. A root hair assay protocol for the monocotyledonous model species Brachypodium distachyon is also included.
Sponsorship
Irish Research Council for Science, Engineering and Technology
Type of Material
Book Chapter
Publisher
Humana Press and Springer
Start Page
73
End Page
82
Copyright (Published Version)
2015 Springer
Language
English
Status of Item
Peer reviewed
Part of
Estevez JM. (ed.). Plant Cell Expansion. Methods in Molecular Biology (Methods and Protocols), vol 1242
ISBN
9781493919017
This item is made available under a Creative Commons License
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