Carbon nanohorn modified platinum electrodes for improved immobilisation of enzyme in the design of glutamate biosensors

DC FieldValueLanguage
dc.contributor.authorFord, Rochelle-
dc.contributor.authorDevereux, Stephen J.-
dc.contributor.authorQuinn, Susan J.-
dc.contributor.authorO'Neill, Robert D.-
dc.date.accessioned2020-09-29T09:32:04Z-
dc.date.available2020-09-29T09:32:04Z-
dc.date.copyright2019 The Royal Society of Chemistryen_US
dc.date.issued2019-07-25-
dc.identifier.citationAnalysten_US
dc.identifier.issn0003-2654-
dc.identifier.urihttp://hdl.handle.net/10197/11597-
dc.description.abstractElectrochemical enzymatic biosensors are the subject of research due to their potential for in vivo monitoring of glutamate, which is a key neurotransmitter whose concentration is related to healthy brain function. This study reports the use of biocompatible oxidised carbon nanohorns (o-CNH) with a high surface area, to enhance the immobilization of glutamate oxidase (GluOx) for improved biosensor performance. Two families of biosensors were designed to interact with the anionic GluOx. Family-1 consists of covalently functionalised o-CNH possessing hydrazide (HYZ) and amine (PEG-NH2) terminated surfaces and Family-2 comprised non-covalently functionalised o-CNH with different loadings of polyethyleneimine (PEI) to form a cationic hybrid. Amperometric detection of H2O2 formed by enzymatic oxidation of glutamate revealed a good performance from all designs with the most improved performance by the PEI hybrid systems. The best response was from a o-CNH:PEI ratio of 1:10 mg mL-1, which yielded a glutamate calibration plateau, JMAX, of 55 ± 9 μA cm-2 and sensitivity of 111 ± 34 μA mM-1 cm-2. The low KM of 0.31 ± 0.05 mM indicated the retention of the enzyme function, and a limit of detection of 0.02 ± 0.004 μM and a response time of 0.88 ± 0.13 s was determined. The results demonstrate the high sensitivity of these biosensors and their potential for future use for the detection of glutamate in vivo.en_US
dc.description.sponsorshipIrish Research Councilen_US
dc.description.sponsorshipUniversity College Dublinen_US
dc.format.mediumPrint-
dc.language.isoenen_US
dc.publisherThe Royal Society of Chemistryen_US
dc.subjectAmino acid oxidoreductasesen_US
dc.subjectBiosensing techniquesen_US
dc.subjectCarbonen_US
dc.subjectElectrochemical techniquesen_US
dc.subjectElectrodesen_US
dc.subjectImmobilized enzymesen_US
dc.subjectGlutamic aciden_US
dc.subjectHydrogen peroxideen_US
dc.subjectLimit of detectionen_US
dc.subjectNanostructuresen_US
dc.subjectOxidation-reductionen_US
dc.subjectPlatinumen_US
dc.subjectPolyethyleneimineen_US
dc.titleCarbon nanohorn modified platinum electrodes for improved immobilisation of enzyme in the design of glutamate biosensorsen_US
dc.typeJournal Articleen_US
dc.internal.authorcontactothersusan.quinn@ucd.ieen_US
dc.statusPeer revieweden_US
dc.identifier.volume144en_US
dc.identifier.issue17en_US
dc.identifier.startpage5299en_US
dc.identifier.endpage5307en_US
dc.identifier.doi10.1039/c9an01085h-
dc.neeo.contributorFord|Rochelle|aut|-
dc.neeo.contributorDevereux|Stephen J.|aut|-
dc.neeo.contributorQuinn|Susan J.|aut|-
dc.neeo.contributorO'Neill|Robert D.|aut|-
dc.date.updated2020-09-14T09:48:35Z-
dc.identifier.grantidGOIPG/2016/805, SJD-
dc.rights.licensehttps://creativecommons.org/licenses/by-nc-nd/3.0/ie/en
item.fulltextWith Fulltext-
item.grantfulltextopen-
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