Monitoring guanine photo-oxidation by enantiomerically resolved Ru(II) dipyridophenazine complexes using inosine-substituted oligonucleotides

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Title: Monitoring guanine photo-oxidation by enantiomerically resolved Ru(II) dipyridophenazine complexes using inosine-substituted oligonucleotides
Authors: Keane, Páraic M.Poynton, Fergus E.Hall, James P.Quinn, Susan J.et al.
Permanent link: http://hdl.handle.net/10197/11606
Date: 1-Oct-2015
Online since: 2020-09-29T15:56:27Z
Abstract: The intercalating [Ru(TAP)2(dppz)]2+ complex can photo-oxidise guanine in DNA, although in mixed-sequence DNA it can be difficult to understand the precise mechanism due to uncertainties in where and how the complex is bound. Replacement of guanine with the less oxidisable inosine (I) base can be used to understand the mechanism of electron transfer (ET). Here the ET has been compared for both Λ- and Δ-enantiomers of [Ru(TAP)2(dppz)]2+ in a set of sequences where guanines in the readily oxidisable GG step in {TCGGCGCCGA}2 have been replaced with I. The ET has been monitored using picosecond and nanosecond transient absorption and picosecond time-resolved IR spectroscopy. In both cases inosine replacement leads to a diminished yield, but the trends are strikingly different for Λ- and Δ-complexes.
Funding Details: Irish Research Council
Science Foundation Ireland
metadata.dc.description.othersponsorship: Royal Irish Academy/Royal Society exchange programme
UK Biotechnology and Biological Sciences Research Council
UK Science and Technology Facilities Council
School of Chemistry and Chemical Biology, UCD
Type of material: Journal Article
Publisher: Royal Society of Chemistry
Journal: Faraday Discussions
Volume: 185
Start page: 455
End page: 469
Copyright (published version): 2015 The Royal Society of Chemistry
Keywords: RutheniumOrganometallic compoundsGuanineInosineOligonucleotidesOxidation-reductionStereoisomerismPhotochemistry
DOI: 10.1039/c5fd00085h
Language: en
Status of Item: Peer reviewed
Appears in Collections:Chemistry Research Collection

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