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Monitoring guanine photo-oxidation by enantiomerically resolved Ru(II) dipyridophenazine complexes using inosine-substituted oligonucleotides
Date Issued
2015-10-01
Date Available
2020-09-29T15:56:27Z
Abstract
The intercalating [Ru(TAP)2(dppz)]2+ complex can photo-oxidise guanine in DNA, although in mixed-sequence DNA it can be difficult to understand the precise mechanism due to uncertainties in where and how the complex is bound. Replacement of guanine with the less oxidisable inosine (I) base can be used to understand the mechanism of electron transfer (ET). Here the ET has been compared for both Λ- and Δ-enantiomers of [Ru(TAP)2(dppz)]2+ in a set of sequences where guanines in the readily oxidisable GG step in {TCGGCGCCGA}2 have been replaced with I. The ET has been monitored using picosecond and nanosecond transient absorption and picosecond time-resolved IR spectroscopy. In both cases inosine replacement leads to a diminished yield, but the trends are strikingly different for Λ- and Δ-complexes.
Sponsorship
Irish Research Council
Science Foundation Ireland
Other Sponsorship
Royal Irish Academy/Royal Society exchange programme
UK Biotechnology and Biological Sciences Research Council
UK Science and Technology Facilities Council
School of Chemistry and Chemical Biology, UCD
Type of Material
Journal Article
Publisher
Royal Society of Chemistry
Journal
Faraday Discussions
Volume
185
Start Page
455
End Page
469
Copyright (Published Version)
2015 The Royal Society of Chemistry
Language
English
Status of Item
Peer reviewed
ISSN
1359-6640
This item is made available under a Creative Commons License
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