Extraction and yield optimisation of fucose, glucans and associated antioxidant activities from laminaria digitata by applying response surface methodology to high intensity ultrasound-assisted extraction

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Title: Extraction and yield optimisation of fucose, glucans and associated antioxidant activities from laminaria digitata by applying response surface methodology to high intensity ultrasound-assisted extraction
Authors: García-Vaquero, MarcoRajauria, GauravTiwari, Brijesh K.Sweeney, TorresO'Doherty, John V.
Permanent link: http://hdl.handle.net/10197/11847
Date: 30-Jul-2018
Online since: 2021-01-18T16:33:22Z
Abstract: The objectives of this study were to employ response surface methodology (RSM) to investigate and optimize the effect of ultrasound-assisted extraction (UAE) variables, temperature, time and amplitude on the yields of polysaccharides (fucose and total glucans) and antioxidant activities (ferric reducing antioxidant power (FRAP) and 1,1-diphenyl-2-picryl-hydrazyl radical scavenging activity (DPPH)) from Laminaria digitata, and to explore the suitability of applying the optimum UAE conditions for L. digitata to other brown macroalgae (L. hyperborea and Ascophyllum nodosum). The RSM with three-factor, four-level Box-Behnken Design (BBD) was used to study and optimize the extraction variables. A second order polynomial model fitted well to the experimental data with R2 values of 0.79, 0.66, 0.64, 0.73 for fucose, total glucans, FRAP and DPPH, respectively. The UAE parameters studied had a significant influence on the levels of fucose, FRAP and DPPH. The optimised UAE conditions (temperature = 76 ◦C, time = 10 min and amplitude = 100%) achieved yields of fucose (1060.7 ± 70.6 mg/100 g dried seaweed (ds)), total glucans (968.6 ± 13.3 mg/100 g ds), FRAP (8.7 ± 0.5 µM trolox/mg freeze-dried extract (fde)) and DPPH (11.0 ± 0.2%) in L. digitata. Polysaccharide rich extracts were also attained from L. hyperborea and A. nodosum with variable results when utilizing the optimum UAE conditions for L. digitata.
Funding Details: Science Foundation Ireland
Type of material: Journal Article
Publisher: MDPI
Journal: Marine Drugs
Volume: 16
Issue: 8
Copyright (published version): 2018 the Authors
Keywords: LaminariaSeaweedPhenolsGlucansFucosePolysaccharidesFree radical scavengersChemical fractionationUltrasonic waves
DOI: 10.3390/md16080257
Language: en
Status of Item: Peer reviewed
ISSN: 1660-3397
This item is made available under a Creative Commons License: https://creativecommons.org/licenses/by-nc-nd/3.0/ie/
Appears in Collections:Agriculture and Food Science Research Collection

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