Nanoparticles Can Wrap Epithelial Cell Membranes and Relocate Them Across the Epithelial Cell Layer

Title: Nanoparticles Can Wrap Epithelial Cell Membranes and Relocate Them Across the Epithelial Cell Layer
Authors: Urbančič, IztokGarvas, MajaKokot, BoštjanCassidy, HilaryMatallanas, Davidet al.
Permanent link: http://hdl.handle.net/10197/11886
Date: 24-Jul-2018
Online since: 2021-01-25T16:00:28Z
Abstract: Although the link between the inhalation of nanoparticles and cardiovascular disease is well established, the causal pathway between nanoparticle exposure and increased activity of blood coagulation factors remains unexplained. To initiate coagulation tissue factor bearing epithelial cell membranes should be exposed to blood, on the other side of the less than a micrometre thin air-blood barrier. For the inhaled nanoparticles to promote coagulation, they need to bind lung epithelial-cell membrane parts and relocate them into the blood. To assess this hypothesis, we use advanced microscopy and spectroscopy techniques to show that the nanoparticles wrap themselves with epithelial-cell membranes, leading to the membrane's disruption. The membrane-wrapped nanoparticles are then observed to freely diffuse across the damaged epithelial cell layer relocating epithelial cell membrane parts over the epithelial layer. Proteomic analysis of the protein content in the nanoparticles wraps/corona finally reveals the presence of the coagulation-initiating factors, supporting the proposed causal link between the inhalation of nanoparticles and cardiovascular disease.
Funding Details: European Commission Horizon 2020
Wellcome Trust
Funding Details: Slovenian Research Agency
Wolfson Foundation
Oxford-internal funding
Type of material: Journal Article
Publisher: American Chemical Society
Journal: Nano Letters
Volume: 18
Issue: 8
Start page: 5294
End page: 5305
Copyright (published version): 2018 American Chemical Society
Keywords: LungCell membraneTitaniumLipid bilayersProteomeSignal transductionCell movementCell survivalBlood coagulationParticle sizeSurface propertiesNanotubesProtein corona
DOI: 10.1021/acs.nanolett.8b02291
Language: en
Status of Item: Peer reviewed
ISSN: 1530-6984
This item is made available under a Creative Commons License: https://creativecommons.org/licenses/by/3.0/ie/
Appears in Collections:SBI Research Collection
Medicine Research Collection

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