A Three-Dimensional Cell Culture Platform for Long Time-Scale Observations of Bio-Nano Interactions

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Title: A Three-Dimensional Cell Culture Platform for Long Time-Scale Observations of Bio-Nano Interactions
Authors: Muraca, FrancescoAlahmari, AmirahGiannone, Valeria A.Adumeau, LaurentYan, YanMcCafferty, Mura M.Dawson, Kenneth A.
Permanent link: http://hdl.handle.net/10197/12162
Date: 4-Nov-2019
Online since: 2021-05-18T11:24:32Z
Abstract: We know surprisingly little about the long-term outcomes for nanomaterials interacting with organisms. To date, most of what we know is derived from in vivo studies that limit the range of materials studied and the scope of advanced molecular biology tools applied. Long-term in vitro nanoparticle studies are hampered by a lack of suitable models, as standard cell culture techniques present several drawbacks, while technical limitations render current three-dimensional (3D) cellular spheroid models less suited. Now, by controlling the kinetic processes of cell assembly and division in a non-Newtonian culture medium, we engineer reproducible cell clusters of controlled size and phenotype, leading to a convenient and flexible long-term 3D culture that allows nanoparticle studies over many weeks in an in vitro setting. We present applications of this model for the assessment of intracellular polymeric and silica nanoparticle persistence and found that hydrocarbon-based polymeric nanoparticles undergo no apparent degradation over long time periods with no obvious biological impact, while amorphous silica nanoparticles degrade at different rates over several weeks, depending on their synthesis method.
Funding Details: Irish Research Council
Funding Details: Princess Nourah Bint Abdulrahman University Project
Type of material: Journal Article
Publisher: ACS
Journal: ACS Nano
Volume: 13
Issue: 11
Start page: 13524
End page: 13536
Copyright (published version): 2019 American Chemical Society
Keywords: Three-dimensional cell cultureCell cycleQuiescenceBio-nano interactionsNanoparticle persistenceNanoparticle degradationSilica nanoparticles
DOI: 10.1021/acsnano.9b07453
Language: en
Status of Item: Peer reviewed
ISSN: 1936-0851
This item is made available under a Creative Commons License: https://creativecommons.org/licenses/by-nc-nd/3.0/ie/
Appears in Collections:Centre for Bionano Interactions (CBNI) Research Collection
Chemistry Research Collection

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