Differential Recognition of Nanoparticle Protein Corona and Modified Low-Density Lipoprotein by Macrophage Receptor with Collagenous Structure

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Title: Differential Recognition of Nanoparticle Protein Corona and Modified Low-Density Lipoprotein by Macrophage Receptor with Collagenous Structure
Authors: Lara, SandraPerez-Potti, AndréHerda, Luciana M.Adumeau, LaurentDawson, Kenneth A.Yan, Yan
Permanent link: http://hdl.handle.net/10197/12168
Date: 18-Apr-2018
Online since: 2021-05-18T14:22:17Z
Abstract: Key practical challenges such as understanding the immunological processes at the nanoscale and controlling the targeting and accumulation of nano-objects in vivo now further stimulate efforts to underpin phenomenological knowledge of the nanoscale with more mechanistic and molecular insight. Thus, the question as to what constitutes nanoscale biological identity continues to evolve. Certainly nanoparticles in contact with a complex biological milieu develop a biological identity, differing from the original nanomaterial, now referred to as the "biomolecular corona". However, this surface-adsorbed layer of biomolecules may in some circumstance lead to different forms of receptor-particle interactions not evident only from the identity of the surface-adsorbed biomolecules and hard to predict or detect by current physicochemical methods. Here we show that scavenger receptors may recognize complex as yet unidentified biomolecular surface layer motifs, even when no current physicochemical analysis is capable of doing so. For instance, fluorescently labeled SiO nanoparticles in a biological milieu are strongly recognized by the macrophage receptor with collagenous structure (MARCO) in even dense biological media (human serum) apparently using a form of binding with which most of the MARCO's known ligands (e.g., LPS, modified LDL) fail to compete. Such observations may suggest the need for a much stronger emphasis on nanoscale receptor-corona and other biomolecular interaction studies if one wishes to unravel how biomolecular recognition drives outcomes in the nanoscale biological domain. 2
Funding Details: European Commission - Seventh Framework Programme (FP7)
Science Foundation Ireland -- replace
Type of material: Journal Article
Publisher: ACS
Journal: ACS Nano
Volume: 12
Issue: 5
Start page: 4930
End page: 4937
Copyright (published version): 2018 ACS
Keywords: AnimalsCattleHumansSilicon dioxideLipoproteinsBovine serum albuminImmunologic receptorsTransfectionGene expressionAdsorptionSurface propertiesNanoparticlesHEK293 cellsProtein unfoldingProtein coronaProtein domains
DOI: 10.1021/acsnano.8b02014
Language: en
Status of Item: Peer reviewed
ISSN: 1936-0851
This item is made available under a Creative Commons License: https://creativecommons.org/licenses/by-nc-nd/3.0/ie/
Appears in Collections:Centre for Bionano Interactions (CBNI) Research Collection
Conway Institute Research Collection
Chemistry Research Collection

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