In depth characterisation of the biomolecular coronas of polymer coated inorganic nanoparticles with differential centrifugal sedimentation

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Title: In depth characterisation of the biomolecular coronas of polymer coated inorganic nanoparticles with differential centrifugal sedimentation
Authors: Perez-Potti, AndréLopez, HenderPelaz, BeatrizKelly, PhilipDawson, Kenneth A.Krpetic, ZeljkaMonopoli, Marco P.et al.
Permanent link: http://hdl.handle.net/10197/12169
Date: 19-Mar-2021
Online since: 2021-05-18T14:46:49Z
Abstract: Advances in nanofabrication methods have enabled the tailoring of new strategies towards the controlled production of nanoparticles with attractive applications in healthcare. In many cases, their characterisation remains a big challenge, particularly for small-sized functional nanoparticles of 5 nm diameter or smaller, where current particle sizing techniques struggle to provide the required sensitivity and accuracy. There is a clear need for the development of new reliable characterisation approaches for the physico-chemical characterisation of nanoparticles with significant accuracy, particularly for the analysis of the particles in the presence of complex biological fluids. Herein, we show that the Differential Centrifugal Sedimentation can be utilised as a high-precision tool for the reliable characterisation of functional nanoparticles of different materials. We report a method to correlate the sedimentation shift with the polymer and biomolecule adsorption on the nanoparticle surface, validating the developed core-shell model. We also highlight its limit when measuring nanoparticles of smaller size and the need to use several complementary methods when characterising nanoparticle corona complexes.
Funding Details: European Commission Horizon 2020
European Commission - Seventh Framework Programme (FP7)
Irish Research Council
Funding Details: Deutsche Forschungsgemeinschaft (DFG)
Xunta de Galicia
RyC program
RCSI StAR programme
Type of material: Journal Article
Publisher: Springer
Journal: Scientific Reports
Volume: 11
Issue: 1
Keywords: NanofabricationNanoparticlesMedical devicesDifferential centrifugal sedimentation (DCS)Biomolecular corona validation
DOI: 10.1038/s41598-021-84029-8
Language: en
Status of Item: Peer reviewed
ISSN: 2045-2322
This item is made available under a Creative Commons License: https://creativecommons.org/licenses/by/3.0/ie/
Appears in Collections:Centre for Bionano Interactions (CBNI) Research Collection
Chemistry Research Collection

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