Mapping protein binding sites on the biomolecular corona of nanoparticles

Title: Mapping protein binding sites on the biomolecular corona of nanoparticles
Authors: Kelly, PhilipÅberg, ChristofferPolo, EsterO'Connell, AnnCookman, JenniferFallon, JonathanKrpetic, ZeljkaDawson, Kenneth A.
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Date: 30-Mar-2015
Online since: 2021-05-18T14:55:45Z
Abstract: Nanoparticles in a biological milieu are known to form a sufficiently long-lived and well-organized 'corona' of biomolecules to confer a biological identity to the particle. Because this nanoparticle-biomolecule complex interacts with cells and biological barriers, potentially engaging with different biological pathways, it is important to clarify the presentation of functional biomolecular motifs at its interface. Here, we demonstrate that by using antibody-labelled gold nanoparticles, differential centrifugal sedimentation and various imaging techniques it is possible to identify the spatial location of proteins, their functional motifs and their binding sites. We show that for transferrin-coated polystyrene nanoparticles only a minority of adsorbed proteins exhibit functional motifs and the spatial organization appears random, which is consistent, overall, with a stochastic and irreversible adsorption process. Our methods are applicable to a wide array of nanoparticles and can offer a microscopic molecular description of the biological identity of nanoparticles.
Funding Details: European Commission - Seventh Framework Programme (FP7)
Irish Research Council
Science Foundation Ireland -- replace
Type of material: Journal Article
Publisher: Springer Nature
Journal: Nature Nanotechnology
Volume: 10
Issue: 5
Start page: 472
End page: 479
Copyright (published version): 2015 Macmillan
Keywords: HumansGoldPolystyrenesProteinsTransferrinTransferrin receptorsEpitopesImmunohistochemistryBinding SitesProtein bindingNanotechnologyMetal nanoparticles
DOI: 10.1038/nnano.2015.47
Language: en
Status of Item: Peer reviewed
ISSN: 1748-3387
This item is made available under a Creative Commons License:
Appears in Collections:Centre for Bionano Interactions (CBNI) Research Collection
Chemistry Research Collection

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