Imaging approach to mechanistic study of nanoparticle interactions with the blood-brain barrier

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Title: Imaging approach to mechanistic study of nanoparticle interactions with the blood-brain barrier
Authors: Bramini, MattiaYe, DongHallerbach, AnnaNic Raghnaill, MichelleSalvati, AnnaÅberg, ChristofferDawson, Kenneth A.
Permanent link: http://hdl.handle.net/10197/12171
Date: 27-May-2014
Online since: 2021-05-18T15:03:36Z
Abstract: Understanding nanoparticle interactions with the central nervous system, in particular the blood-brain barrier, is key to advances in therapeutics, as well as assessing the safety of nanoparticles. Challenges in achieving insights have been significant, even for relatively simple models. Here we use a combination of live cell imaging and computational analysis to directly study nanoparticle translocation across a human in vitro blood-brain barrier model. This approach allows us to identify and avoid problems in more conventional inferential in vitro measurements by identifying the catalogue of events of barrier internalization and translocation as they occur. Potentially this approach opens up the window of applicability of in vitro models, thereby enabling in depth mechanistic studies in the future. Model nanoparticles are used to illustrate the method. For those, we find that translocation, though rare, appears to take place. On the other hand, barrier uptake is efficient, and since barrier export is small, there is significant accumulation within the barrier. © 2014 American Chemical Society.
Funding Details: European Commission - Seventh Framework Programme (FP7)
Science Foundation Ireland -- replace
Funding Details: Irish Government’s Programme for Research in Third Level Institutions
Type of material: Journal Article
Publisher: ACS
Journal: ACS Nano
Volume: 8
Issue: 5
Start page: 4304
End page: 4312
Copyright (published version): 2014 ACS
Keywords: Blood-brain barrierCapillariesBrainCell LineLysosomesHumansCollagenDrug carriersCulture mediaConfocal microscopyFluorescence microscopyTemperatureNanotechnologyComputer simulationNanoparticlesTranscytosis
DOI: 10.1021/nn5018523
Language: en
Status of Item: Peer reviewed
ISSN: 1936-0851
This item is made available under a Creative Commons License: https://creativecommons.org/licenses/by-nc-nd/3.0/ie/
Appears in Collections:Centre for Bionano Interactions (CBNI) Research Collection
Chemistry Research Collection

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