Understanding intracellular nanoparticle trafficking fates through spatiotemporally resolved magnetic nanoparticle recovery

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Title: Understanding intracellular nanoparticle trafficking fates through spatiotemporally resolved magnetic nanoparticle recovery
Authors: Sheridan, EmilyVercellino, SilviaCursi, LorenzoAdumeau, LaurentBehan, James A.Dawson, Kenneth A.
Permanent link: http://hdl.handle.net/10197/12172
Date: 3-Mar-2021
Online since: 2021-05-18T15:29:55Z
Abstract: The field of nanomedicine has the potential to be a game-changer in global health, with possible applications in prevention, diagnostics, and therapeutics. However, despite extensive research focus and funding, the forecasted explosion of novel nanomedicines is yet to materialize. We believe that clinical translation is ultimately hampered by a lack of understanding of how nanoparticles really interact with biological systems. When placed in a biological environment, nanoparticles adsorb a biomolecular layer that defines their biological identity. The challenge for bionanoscience is therefore to understand the evolution of the interactions of the nanoparticle–biomolecules complex as the nanoparticle is trafficked through the intracellular environment. However, to progress on this route, scientists face major challenges associated with isolation of specific intracellular compartments for analysis, complicated by the diversity of trafficking events happening simultaneously and the lack of synchronization between individual events. In this perspective article, we reflect on how magnetic nanoparticles can help to tackle some of these challenges as part of an overall workflow and act as a useful platform to investigate the bionano interactions within the cell that contribute to this nanoscale decision making. We discuss both established and emerging techniques for the magnetic extraction of nanoparticles and how they can potentially be used as tools to study the intracellular journey of nanomaterials inside the cell, and their potential to probe nanoscale decision-making events. We outline the inherent limitations of these techniques when investigating particular bio-nano interactions along with proposed strategies to improve both specificity and resolution. We conclude by describing how the integration of magnetic nanoparticle recovery with sophisticated analysis at the single-particle level could be applied to resolve key questions for this field in the future.
Funding Details: Irish Research Council
Science Foundation Ireland -- replace default
Funding Details: Celtic Advanced Life Science Innovation Network (CALIN)
Ireland-Wales Programme
UCD School of Biomolecular and Biomedical Science
Type of material: Journal Article
Publisher: Royal Society of Chemistry
Journal: Nanoscale Advances
Volume: 3
Issue: 9
Start page: 2397
End page: 2410
Copyright (published version): 2021 the Authors
Keywords: NanoparticlesMedical devicesCorona formationMagnetic extraction
DOI: 10.1039/d0na01035a
Language: en
Status of Item: Peer reviewed
ISSN: 2516-0230
This item is made available under a Creative Commons License: https://creativecommons.org/licenses/by-nc-nd/3.0/ie/
Appears in Collections:Centre for Bionano Interactions (CBNI) Research Collection
Conway Institute Research Collection
Chemistry Research Collection

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